Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose

Applied and Environmental Microbiology
Soo-Jin YeomDeok-Kun Oh

Abstract

The uncharacterized gene previously proposed as a mannose-6-phosphate isomerase from Bacillus subtilis was cloned and expressed in Escherichia coli. The maximal activity of the recombinant enzyme was observed at pH 7.5 and 40 degrees C in the presence of 0.5 mM Co(2+). The isomerization activity was specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the d and l forms of ribose, lyxose, talose, mannose, and allose. The enzyme exhibited the highest activity for l-ribulose among all pentoses and hexoses. Thus, L-ribose, as a potential starting material for many L-nucleoside-based pharmaceutical compounds, was produced at 213 g/liter from 300-g/liter L-ribulose by mannose-6-phosphate isomerase at 40 degrees C for 3 h, with a conversion yield of 71% and a volumetric productivity of 71 g liter(-1) h(-1).

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Citations

Jun 10, 2011·Applied Microbiology and Biotechnology·Yu-Ri Lim, Deok-Kun Oh
Aug 30, 2011·Applied Microbiology and Biotechnology·Chao HuCuiying Hu
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hiromi YoshidaShigehiro Kamitori
Oct 5, 2014·Applied Microbiology and Biotechnology·Zheng XuHong Xu
Jun 15, 2010·Journal of Bioscience and Bioengineering·Hyun-Jung KwonDeok-Kun Oh
Jul 8, 2015·Applied Microbiology and Biotechnology·Wanmeng MuBo Jiang
Jul 10, 2010·Journal of Molecular Biology·Laura M van StaalduinenZongchao Jia
Jun 28, 2016·Applied Microbiology and Biotechnology·Ziwei ChenWanmeng Mu
Feb 29, 2016·Applied Biochemistry and Biotechnology·Manisha J PatelDarshan H Patel
Mar 23, 2017·Journal of Bacteriology·Anthony M Sperber, Jennifer K Herman
Feb 3, 2018·Applied Microbiology and Biotechnology·Jiawei HuangWanmeng Mu
May 7, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu
Mar 27, 2018·Applied Microbiology and Biotechnology·Ziwei ChenWanmeng Mu
Feb 24, 2020·Applied Microbiology and Biotechnology·Ming ChenWanmeng Mu
Jul 4, 2018·Applied Microbiology and Biotechnology·Zheng FangWanmeng Mu
Jun 26, 2020·The Journal of Microbiology·Soo-Jung KimSoo-Jin Yeom
Jun 4, 2020·Journal of Agricultural and Food Chemistry·Zijie LiXiao-Dong Gao

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