Substrate specificity of a recombinant D-lyxose isomerase from Providencia stuartii for monosaccharides

Journal of Bioscience and Bioengineering
Hyun-Jung KwonDeok-Kun Oh

Abstract

The specific activity and catalytic efficiency (k(cat)/K(m)) of the recombinant putative protein from Providencia stuartii was the highest for D-lyxose among the aldose substrates, indicating that it is a D-lyxose isomerase. Gel filtration analysis suggested that the native enzyme is a dimer with a molecular mass of 44 kDa. The maximal activity for D-lyxose isomerization was observed at pH 7.5 and 45 degrees C in the presence of 1 mM Mn(2+). The enzyme exhibited high isomerization activity for aldose substrates with the C2 and C3 hydroxyl groups in the left-hand configuration, such as D-lyxose, D-mannose, L-ribose, D-talose, and L-allose (listed in decreasing order of activity). The enzyme exhibited the highest activity for D-xylulose among all pentoses and hexoses. Thus, D-lyxose was produced at 288 g/l from 500 g/l D-xylulose by D-lyxose isomerase at pH 7.5 and 45 degrees C for 2 h, with a conversion yield of 58% and a volumetric productivity of 144 g l(-1) h(-1). The observed k(cat)/K(m) (920 mM(-1) s(-1)) of P. stuartiid-lyxose isomerase for D-xylulose is higher than any of the k(cat)/K(m) values previously reported for sugar and sugar phosphate isomerases with monosaccharide substrates. These results suggest that the enzym...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L DoongY C Cheng
Dec 1, 1996·Glycobiology·S MuniruzzamanA D Elbein
May 25, 2002·Journal of Nutritional Science and Vitaminology·Tatsuhiro MatsuoKen Izumori
Jun 6, 2002·Die Naturwissenschaften·Ken Izumori
Jan 4, 2003·Journal of Medicinal Food·Gilbert V Levin
Jul 9, 2004·Applied and Environmental Microbiology·Joo-Heon Park, Carl A Batt
Dec 26, 2006·Journal of Bacteriology·Eun-Ah ChoYu-Ryang Pyun
Nov 6, 2008·Journal of Biotechnology·Ran-Young YoonDeok-Kun Oh
Jan 23, 2009·Applied Microbiology and Biotechnology·Ran-Young YoonDeok-Kun Oh

Citations

Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Koen BeerensWim Soetaert
Jul 8, 2015·Applied Microbiology and Biotechnology·Wanmeng MuBo Jiang
Feb 3, 2018·Applied Microbiology and Biotechnology·Jiawei HuangWanmeng Mu
Nov 1, 2012·Perspectives in Public Health·Shariful ShikderAndrew Price
Jul 4, 2018·Applied Microbiology and Biotechnology·Zheng FangWanmeng Mu
Oct 23, 2019·Applied Microbiology and Biotechnology·Hao WuWanmeng Mu

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.