Substrate specificity of chitinases from two species of fish, greenling, Hexagrammos otakii, and common mackerel, Scomber japonicus, and the insect, tobacco hornworm, Manduca sexta

Bioscience, Biotechnology, and Biochemistry
Masahiro MatsumiyaKarl J Kramer

Abstract

Three chitinase isozymes, HoChiA, HoChiB, and HoChiC, were purified from the stomach of the greenling, Hexagrammos otakii, by ammonium sulfate fractionation, followed by column chromatography on Chitopearl Basic BL-03 and CM-Toyopearl 650S. The molecular masses and pIs of HoChiA, HoChiB, and HoChiC are 62 kDa and pH 5.7, 51 kDa and pH 7.6, and 47 kDa and pH 8.8, respectively. Substrate specificities of these chitinases were compared with those of another fish stomach chitinase from the common mackerel, Scomber japonicus (SjChi), as well as two from the tobacco hornworm, Manduca sexta (MsChi535 and MsChi386). The efficiency parameters, kcat/Km, toward glycolchitin for HoChiA and SjChi were larger than those for HoChiB and HoChiC. The relative activities of HoChiA and SjChi toward various forms of chitin were as follows: shrimp shell or crab shell alpha-chitin > beta-chitin > silkworm cuticle alpha-chitin. On the other hand, the relative activities of HoChiB and HoChiC were beta-chitin > silkworm alpha-chitin > shrimp and crab alpha-chitin. MsChi535 preferred silkworm alpha-chitin to shrimp and crab alpha-chitins, and no activity was observed toward beta-chitin. MsChi386, which lacked the C-terminal linker region and the chitin-b...Continue Reading

References

Feb 1, 1994·International Journal of Biological Macromolecules·Y ShigemasaH Saimoto
Jan 1, 1993·The Plant Journal : for Cell and Molecular Biology·D B CollingeK Vad
Aug 1, 1997·Insect Biochemistry and Molecular Biology·D KogaY Nagamatsu
Mar 8, 2000·International Journal of Biological Macromolecules·M ZhangS Hirano
Apr 20, 2000·Enzyme and Microbial Technology·R S PatilM V Deshpande
Jul 25, 2000·EXS·R A Muzzarelli
Jul 25, 2000·EXS·D KogaM Matsumiya
Nov 22, 2000·The Journal of Biological Chemistry·R G BootJ M Aerts
Jul 20, 2002·Zoological Science·Hiroyuki OshimaSakae Kikuyama
Oct 9, 2002·Current Protein & Peptide Science·T Fukamizo
May 29, 2003·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneKarl J Kramer
Jun 25, 2004·Bioscience, Biotechnology, and Biochemistry·Shuji KarasudaDaizo Koga

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Citations

Dec 9, 2008·Bioscience, Biotechnology, and Biochemistry·Kazuya ShirotaMasahiro Matsumiya
Apr 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qingsong ZhuSubbaratnam Muthukrishnan
Mar 20, 2009·Protein Expression and Purification·Mana IkedaMasahiro Matsumiya
Apr 4, 2017·International Journal of Biological Macromolecules·Mana IkedaMasahiro Matsumiya
Aug 2, 2007·Physiological Genomics·Svetlana KalujnaiaGordon Cramb

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