Substrate specificity of IgGs with peroxidase and oxidoreductase activities from sera of patients with systemic lupus erythematosus and multiple sclerosis

Journal of Molecular Recognition : JMR
Anna S TolmachevaGeorgy A Nevinsky

Abstract

The analysis of IgGs to protect humans from oxidative stress through oxidation of harmful compounds was carried out. We have compared here for the first time peroxidase (in the presence of H2 O2 ) and oxidoreductase (in the absence of H2 O2 ) activities of IgGs from sera of healthy humans and patients with systemic lupus erythematosus (SLE) and multiple sclerosis (MS). In addition, substrate specificity of SLE and MS IgG preparations in the oxidation of different compounds was analyzed: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 3,3'-diaminobenzidine (DAB), homovanillic acid (HVA), o-phenylenediamine (OPD), α-naphthol, 3-amino-9-ethylcarbazole (AEC), p-hydroquinone (pHQ), and adrenaline. IgGs of healthy humans and SLE and MS patients oxidized DAB, ABTS, and OPD due to their peroxidase and oxidoreductase activities, while other compounds were substrates of IgGs only in the presence of H2 O2 : adrenaline was not oxidized by both activities of IgGs. The average SLE IgGs peroxidase activity increased statistically significant in comparison with abzymes from healthy humans in the order (-fold): OPD (1.2) <  DAB (1.7) < α-naphtol (2.2) ≤ AEC (2.4) < ABTS (4.5) < 5-ASA (10.6), while with oxidoreductase activity: OP...Continue Reading

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May 7, 2015·Journal of Molecular Recognition : JMR·Anna S TolmachevaGeorgy A Nevinsky
Jan 31, 2017·Journal of the Neurological Sciences·Ana García-BlancoConsuelo Cháfer-Pericás
Feb 8, 2018·Royal Society Open Science·Anna S TolmachevaGeorgy A Nevinsky

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Citations

Aug 6, 2020·International Journal of Molecular Sciences·Evgeny A ErmakovValentina N Buneva
Mar 21, 2021·Journal of Dairy Science·Georgy A NevinskyNatalia I Menzorova
Apr 4, 2021·International Journal of Molecular Sciences·Evgeny A ErmakovGeorgy A Nevinsky
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna S TolmachevaGeorgy A Nevinsky

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