PMID: 7011412Jan 26, 1981Paper

Substrate specificity of lysophospholipase-transacylase from rat lung and its action on various physical forms of lysophosphatidylcholine

Biochimica Et Biophysica Acta
G P Van HeusdenH van den Bosch

Abstract

Lysophospholipase-transacylase (lysolecithin acylhydrolase, EC 3.1.1.5) from rat lung catalyzes the transfer of acyl groups from lysophosphatidylcholine to either water or another molecule of lysophosphatidylcholine. Studies on the substrate specificity of the purified enzyme showed that a phosphate group in the substrate is essential for enzymatic activity; monoacylglycerol is not hydrolyzed, nor does it serve as an acceptor of acyl groups. The influence of the acyl chain in lysophosphatidylcholine was investigated by using mixtures of differently labelled lysophosphatidylcholine species, or by studying the transfer of [1-14C]Palmitate from [1-14C]palmitoylpropane (1,3)diol-phosphocholine to various 1-acyl-sn-glycero-3-phosphocholines. Lysophosphatidylcholines with acyl chains comprised of ten or more C-atoms were found to serve as acyl acceptors. This finding was used to determine the action of the enzyme on 1-[1-14C]auroyl- and 1[1-14C]myristoyl-sn-glycero-3-phosphocholine both below and above the critical micelle concentration of the substrate. Monomeric substrate was effectively hydrolyzed, but the transacylase activity of the enzyme was only expressed when substrate micelles were present. Likewise, no transacylase activit...Continue Reading

References

Mar 1, 1979·Archives of Biochemistry and Biophysics·Y N LinM I Horowitz
Mar 29, 1979·Biochimica Et Biophysica Acta·A J Aarsman, H van den Bosch
Oct 12, 1979·Biochemical and Biophysical Research Communications·G P van Heusden, H van den Bosch
Feb 28, 1975·Biochimica Et Biophysica Acta·J G ManderslootJ De Gier
Jun 6, 1967·Biochimica Et Biophysica Acta·S A Ibrahim
Jan 1, 1973·Annual Review of Biochemistry·S Gatt, Y Barenholz
Oct 1, 1972·The Tohoku Journal of Experimental Medicine·T AkinoM Abe
May 29, 1974·Biochimica Et Biophysica Acta·H van den BoschL L van Deenen
Oct 22, 1968·Biochimica Et Biophysica Acta·H van den BoschL L van Deenen
Jun 1, 1965·Biochimica Et Biophysica Acta·H van den Bosch, L L van Deenen
Jul 7, 1965·Biochimica Et Biophysica Acta·J F Erbland, G V Marinetti
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Jan 1, 1984·The International Journal of Biochemistry·C CasalsR Arche
Jan 1, 1987·Progress in Lipid Research·J L Harwood
May 29, 2004·Biochimica Et Biophysica Acta·Dennis E Vance, Friedrich Spener
Feb 15, 1983·Archives of Biochemistry and Biophysics·H OkuyamaW E Lands
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Dec 23, 1981·Biochimica Et Biophysica Acta·G P Van HeusdenH Van den Bosch
Mar 1, 1983·Biochimica Et Biophysica Acta·P V ReddyH H Schmid

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