Abstract
Recently, we have discovered site-specific endonucleases, which recognize and cleave only DNA sequences with 5-methylcytosine. Two specificities of such endonucleases have been described. Enzymes BisI, BlsI, and GluI are isoschizomers and hydrolyze the DNA sequence 5'-GCNGC-3'/3'-CGNCG-5', which is methylated in different ways. The enzyme GlaI cleaves the DNA sequence 5'-GCGC-3'/3'-CGCG-5' if there are two, three or four 5-methylcytosines. The goal of the present work is to study in detail the composition of recognition sequence and effect of the methylated cytosines on the efficiency of DNA cleavage by the methyl-directed DNA endonuclease GlaI RESULTS: In a recent work we have studied the dependence of GlaI activity on the quantity and location of 5-methylcytosines in the enzyme recognition sequence 5'-GCGC-3'/3'-CGCG-5'. A significant DNA cleavage has been observed for oligonucleotide duplexes, which include either three or four 5-methylcytosines. In this work we have studied dependence of the GlaI activity on quantity and location of methylated cytosines, as well as on composition of the recognition sequence. The list of good substrates for GlaI includes a fully methylated site 5'-CGCG-3'/3'-GCGC-5', sites with three cytosin...Continue Reading
References
Jan 5, 1978·Journal of Molecular Biology·A P Bird, E M Southern
Jan 5, 1978·Journal of Molecular Biology·A P Bird
Jan 1, 1969·Annual Review of Biochemistry·W Arber, S Linn
Sep 15, 2001·Nucleic Acids Research·A Pingoud, A Jeltsch
Mar 26, 2003·Nucleic Acids Research·Richard J RobertsShuang-yong Xu
Sep 7, 2004·Expert Review of Molecular Diagnostics·Florian EckhardtKurt Berlin
Apr 26, 2006·Electrophoresis·Tomoko TakamiyaHisato Okuizumi
Citations
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lisa S ShockShirley M Taylor
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Devora Cohen-KarniYu Zheng
Nov 10, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Ken IshikawaIchizo Kobayashi
Feb 9, 2010·Nucleic Acids Research·Robert J WoodPeter L Roach
May 7, 2010·Nucleic Acids Research·Yu ZhengRichard J Roberts
Mar 23, 2011·Nucleic Acids Research·Shuang-Yong XuPatrick Linder
Sep 12, 2012·Nucleic Acids Research·Keith N RandPeter L Molloy
Aug 31, 2013·Nucleic Acids Research·Wil A M Loenen, Elisabeth A Raleigh
Jul 18, 2009·BMC Genomics·Murat A AbdurashitovSergey Kh Degtyarev
Apr 18, 2012·PloS One·Ali TaheriMargaret Y Gruber
Dec 3, 2011·Expert Review of Molecular Diagnostics·Gerda EggerAndreas Weinhäusel
Jan 12, 2016·Biology·Sergey Kurdyukov, Martyn Bullock
Jul 30, 2008·Protein Expression and Purification·Elizabeth A Mulligan, John J Dunn
Jul 31, 2008·The Plant Journal : for Cell and Molecular Biology·Anuradha SinghPeter Meyer
Jul 22, 2008·Analytical Biochemistry·Alexey FomenkovShuang-yong Xu
Jun 1, 2014·Nucleic Acids Research·Alfred PingoudWolfgang Wende
Nov 28, 2013·Radiation Research·Ji Eun KimJin-Hong Kim
Dec 23, 2014·Epigenomics·Christa NoehammerAndreas Weinhaeusel
Jun 21, 2016·Chemical Reviews·Peter Weigele, Elisabeth A Raleigh
Jun 30, 2016·Scientific Reports·Shuang-Yong XuRichard J Roberts
Jul 5, 2016·Expert Opinion on Therapeutic Patents·Pan XuZhongjie Liang
Feb 24, 2017·Scientific Reports·Sunil Kumar, Yonggyun Kim
Jun 28, 2017·PloS One·Alexey FomenkovElisabeth A Raleigh
Apr 21, 2018·Chemical Science·Yueying SunZhengping Li
Sep 12, 2018·Nucleic Acids Research·Marlena KisialaMatthias Bochtler
Jun 8, 2021·Chemical Science·Li-Juan WangChun-Yang Zhang
Oct 23, 2010·BioTechniques·Keith N Rand, Peter L Molloy