PMID: 6406230Jun 15, 1983Paper

Substrate specificity of the human splenic non-specific soluble beta-glucosidase

European Journal of Biochemistry
A MaretL Douste-Blazy

Abstract

The soluble non-specific 4-methylumbelliferyl-beta-D-glucosidase, one of the three splenic groups previously reported [Maret, A., Salvayre, R., Nègre, A., and Douste-Blazy, L. (1981) Eur. J. Biochem. 115, 455-461], was partially purified by gel filtration and heat-inactivation (which inactivated the contaminating acid beta-galactosidase). Its substrate specificity was accurately demonstrated by comparing the enzymic properties towards each substrate and two-substrate mixed assays: 4-methylumbelliferyl beta-D-galactopyranoside, beta-D-glucopyranoside, beta-D-fucopyranoside, alpha-L-arabinopyranoside and beta-D-xylopyranoside (relative rates 100, 95, 70, 15, 5, respectively in the standard conditions used) were competitively hydrolysed. Information about the enzymic site was given by the effect of various substrate analogs: generally we observed a greater inhibition of beta-galactosidase activity than beta-glucosidase and beta-fucosidase activities. Moreover, p-nitrophenyl-beta-D-mannopyranoside showed a strong inhibitory effect on the three main activities of this enzyme, similar to D-glucono-1,5-lactone inhibition. A selective inhibition by Triton X-100 on beta-D-galactosidase activity and in a lesser degree on beta-D-fucosidas...Continue Reading

References

Jan 23, 1976·Biochimica Et Biophysica Acta·R H GlewA R Christopher
Jan 30, 1977·Archives of Biochemistry and Biophysics·J J Distler, G W Jourdian
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y B ChiaoR H Glew
Jun 6, 1979·Biochimica Et Biophysica Acta·Y Ben-YosephH L Nadler
Aug 1, 1976·Archives of Biochemistry and Biophysics·S P PetersR H Glew
Apr 8, 1976·Biochimica Et Biophysica Acta·M A ChesterP A Ockerman
Jul 1, 1966·The Journal of Clinical Investigation·R O BradyD Shapiro
Jan 1, 1969·Biochimica Et Biophysica Acta·V Patel, A L Tappel
Feb 1, 1980·The Biochemical Journal·M Yaqoob, M Carroll

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Citations

Jun 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N N ChuangK S Lin
Aug 1, 1985·European Journal of Biochemistry·J M AertsA W Schram
May 12, 1988·Biochimica Et Biophysica Acta·M C Sa MirandaA W Schram

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