PMID: 9188804May 22, 1997Paper

Substrate specificity of the mammary tissue anionic amino acid carrier operating in the cotransport and exchange modes

Biochimica Et Biophysica Acta
I D MillarD B Shennan

Abstract

The substrate specificity of the rat mammary tissue high affinity, Na+-dependent anionic amino acid transport system has been investigated using explants and the perfused mammary gland. D-Aspartate appears to be transported via the high affinity, Na+-dependent L-glutamate carrier. Thus, D-aspartate transport by rat mammary tissue was Na+-dependent and saturable with respect to extracellular D-aspartate with a Km and Vmax of 32.4 microM and 49.0 nmol/2 min per g of cells respectively. The uptake of D-aspartate by mammary explants was cis-inhibited by L-glutamate and L-aspartate, but not by D-glutamate. L-glutamate uptake by mammary tissue explants was cis-inhibited by beta-glutamate, L-cysteate, L-cysteine sulfinate and dihydrokainate but not by DL-alpha-aminoadipate. In addition, dihydrokainate, but not DL-alpha-aminoadipate inhibited D-aspartate and L-glutamate uptake by the perfused gland. D-Aspartate efflux from mammary tissue explants was trans-accelerated by external L-glutamate in a dose-dependent fashion (50-500 microM). The effect of L-glutamate on D-aspartate efflux was dependent on the presence of extracellular Na+. D-Aspartate, L-aspartate and L-cysteine sulfinate (at 500 microM) also markedly trans-stimulated D-aspa...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T StorckW Stoffel
Jan 1, 1990·Comparative Biochemistry and Physiology. A, Comparative Physiology·D B Shennan, S A McNeillie
Nov 16, 2001·Biochemical Society Transactions·B I KannerA Bendahan

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Citations

Mar 11, 1998·Acta Physiologica Scandinavica·D T Calvert, D B Shennan
Sep 10, 2014·Amino Acids·Rodrigo ManjarinNathalie L Trottier
Oct 17, 2013·American Journal of Physiology. Cell Physiology·D B Shennan
Apr 6, 2002·American Journal of Physiology. Endocrinology and Metabolism·David A GronebergHannelore Daniel
Apr 5, 2001·American Journal of Physiology. Cell Physiology·T Welbourne, I Nissim
Jul 14, 2000·Physiological Reviews·D B Shennan, M Peaker
Aug 26, 1998·Biochimica Et Biophysica Acta·D B ShennanV A Zammit
Sep 12, 1998·Biochimica Et Biophysica Acta·D B ShennanC A Boyd
Oct 16, 1999·Biochimica Et Biophysica Acta·I D Millar, D B Shennan
Apr 21, 2009·Metabolism: Clinical and Experimental·Gabriela AlemánArmando R Tovar

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