Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del.

Antimicrobial Agents and Chemotherapy
Hyojeong YiHeenam Stanley Kim

Abstract

We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

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Citations

Sep 14, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Katherine A Rhodes, Herbert P Schweizer
Jul 23, 2014·Antimicrobial Agents and Chemotherapy·Junghyun HwangHeenam Stanley Kim
Jun 27, 2020·Journal of Enzyme Inhibition and Medicinal Chemistry·Suwon KimDong Hae Shin
Oct 30, 2015·Clinical Microbiology Reviews·Alain PhilipponRoger Labia

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