Substrate stiffness affects epithelial-mesenchymal transition of cervical cancer cells through miR-106b and its target protein DAB2

International Journal of Oncology
Jinlan PiaoLi Geng

Abstract

The effects of different substrate stiffness were investigated on epithelial-mesenchymal transition (EMT) of cervical cancer cell lines and the role of miR-106b and its target protein DAB2 therein. Cervical cancer cell lines HeLa and SiHa were cultured on artificial substrates with different stiffness prepared using different ratios of acrylamide and bis-acrylamide. Changes of microRNA profiles were detected using microRNA chip analysis, and the expression levels of EMT-related markers E-cadherin and vimentin were detected using western blotting and real-time PCR. In addition, the effects of miR-106b overexpression as well as miR-106b and DAB2 knockdown on expression of E-cadherin and vimentin were also examined using western blotting and real-time PCR. The results showed that i) cervical cancer cell lines SiHa and HeLa cultured on substrate with stiffness of 20 kPa had the strongest EMT ability, showed the highest levels of vimentin and lowest levels of E-cadherin, compared with cells cultured on substrate with stiffness of 1 kPa; ii) miR-106b knockdown reversed the effects of substrate stiffness on EMT of cervical cancer cells, while miR-106 overexpression and DAB2 knockdown induced EMT of cervical cancer cells cultured on su...Continue Reading

References

Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·D M ParkinP Pisani
Oct 5, 2010·PloS One·Robert W TilghmanJ Thomas Parsons
Dec 3, 2010·Breast Cancer Research : BCR·Andrew EvansAlastair Thompson
Mar 16, 2011·Journal of Cell Science·Scott Valastyan, Robert A Weinberg
Apr 28, 2012·BMC Genomics·Nadir YehyaSusan S Margulies
Apr 25, 2013·Cardiovascular Research·Peter NethChristian Weber
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Jul 10, 2014·Reports on Progress in Physics·Claudia Tanja Mierke
Nov 22, 2014·Nature Reviews. Molecular Cell Biology·Caroline BonnansZena Werb
Dec 17, 2014·Annals of Global Health·Jin-Kyoung Oh, Elisabete Weiderpass
Sep 8, 2015·Seminars in Cancer Biology·Anika NagelkerkePaul N Span
Nov 3, 2015·Experimental Cell Research·Jennifer L YoungJoachim P Spatz
Nov 8, 2015·Experimental Cell Research·Aron Parekh, Alissa M Weaver
Nov 26, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jie ZhangJiaxuan Qiu
Nov 26, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mario PoljakKatja Seme
Dec 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xinsheng LiuJun Fang
Mar 16, 2016·BMC Cancer·Oscar Peralta-ZaragozaCarlos Pérez-Plasencia

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Citations

Jul 4, 2020·Journal of Cellular Physiology·María D Vazquez-CarreteroMaría J Peral
Oct 3, 2018·Cell Death Discovery·Yuexiong YiWei Zhang
Jan 5, 2019·Archives of Gynecology and Obstetrics·Shan ZongYing Yue
Mar 16, 2021·Archives of Biochemistry and Biophysics·Abdulghani A KhilanHenning F Horn
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dawid MehlichPaweł K Włodarski

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