Substrate stiffness affects neural network activity in an extracellular matrix proteins dependent manner

Colloids and Surfaces. B, Biointerfaces
Yu-Qiao WenHu-Cheng Zhao

Abstract

Neuronal growth, differentiation, extension, branching and neural network activity are strongly influenced by the mechanical property of extracellular matrix (ECM). However, the mechanism by which substrate stiffness regulates a neural network activity, and the importance of ECM composition in conferring substrate stiffness sensing have not been explored. To address this question, the hippocampal neurons were seeded on the polydimethylsiloxane (PDMS) substrate with different stiffness, which were coated with fibronectin and laminin respectively. Our results show that voltage-gated Ca2+ channel currents are greater in neurons on the stiff substrate than on the soft substrate. In addition, the neurons exhibit a greater increase of Ca2+ currents on laminin-coated stiff substrate than on those coated with fibronectin, indicating that the composition of ECM can modulate responses to substrate stiffness of neurons. Paired patch-clamp recordings have shown that upregulation of neural effective synaptic connectivity is greater on the laminin-coated stiff substrate than on the fibronectin-coated ones. Consistently, laminin-coated stiff substrate enhances Ca2+ oscillations of neurons is greater that compared with the fibronectin-coated o...Continue Reading

Citations

Jan 5, 2019·Bioscience Reports·Yibing WuDeming Zhang
May 2, 2020·The European Journal of Neuroscience·Chloe M HallGraham K Sheridan
Sep 18, 2020·Journal of Neurochemistry·Sercin KarahuseyinogluYasemin Gürsoy-Özdemir
Jan 14, 2021·In Vitro Cellular & Developmental Biology. Animal·Wesley A AndersonMichael J Moore
Jul 8, 2019·ACS Biomaterials Science & Engineering·Yang YuHucheng Zhao
Jan 14, 2022·Biomaterials Science·Alp OzgunFabio Variola

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