Substrate stiffness modulates lung cancer cell migration but not epithelial to mesenchymal transition

Journal of Biomedical Materials Research. Part a
V C ShuklaS N Ghadiali

Abstract

Biomechanical properties of the tumor microenvironment, including matrix/substrate stiffness, play a significant role in tumor evolution and metastasis. Epithelial to Mesenchymal Transition (EMT) is a fundamental biological process that is associated with increased cancer cell migration and invasion. The goal of this study was to investigate (1) how substrate stiffness modulates the migration behaviors of lung adenocarcinoma cells (A549) and (2) if stiffness-induced changes in cell migration correlate with biochemical markers of EMT. Collagen-coated polydimethylsiloxane (PDMS) substrates and an Ibidi migration assay were used to investigate how substrate stiffness alters the migration patterns of A549 cells. RT-PCR, western blotting and immunofluorescence were used to investigate how substrate stiffness alters biochemical markers of EMT, that is, E-cadherin and N-cadherin, and the phosphorylation of focal adhesion proteins. Increases in substrate stiffness led to slower, more directional migration but did not alter the biochemical markers of EMT. Interestingly, growth factor (i.e., Transforming Growth Factor-β) stimulation resulted in similar levels of EMT regardless of substrate stiffness. We also observed decreased levels of ...Continue Reading

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Citations

Feb 12, 2017·Journal of Biomedical Materials Research. Part a·Tingting XiaLi Yang
Mar 15, 2018·Journal of Cell Science·Neha SaxenaShamik Sen
Aug 29, 2018·Journal of Biomedical Materials Research. Part a·Xiaoli XuLiling Tang
May 31, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Shane C AllenLaura J Suggs
Mar 19, 2019·Journal of Biomedical Materials Research. Part a·Shohreh AzadiMajid Ebrahimi Warkiani
Nov 14, 2019·Cellular and Molecular Bioengineering·Steinar Evje, Jahn Otto Waldeland
Jan 2, 2021·Journal of Biomechanics·Victoria L ThaiJ Kent Leach
Nov 15, 2020·Nanomaterials·Indra Van ZundertSusana Rocha
Mar 7, 2021·Sensors·Gabriel A ClarkeNicole N Hashemi
May 2, 2020·The Journal of Physical Chemistry Letters·Jingqiang LiChing-Hwa Kiang
Jun 10, 2019·ACS Biomaterials Science & Engineering·Karolina P ValenteAlexandre G Brolo
Jan 20, 2021·ACS Biomaterials Science & Engineering·Madison N TemplesBlanka Sharma

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