Substrate sustained release-based high efficacy biosynthesis of GABA by Lactobacillus brevis NCL912

Microbial Cell Factories
Qiong WangHaixing Li

Abstract

Gamma-aminobutyric acid (GABA) plays a significant role in the food and drug industries. Our previous study established an efficient fed-batch fermentation process for Lactobacillus brevis NCL912 production of GABA from monosodium L-glutamate; however, monosodium L-glutamate may not be an ideal substrate, as it can result in the rapid increase of pH due to decarboxylation. Thus, in this study, L-glutamic acid was proposed as a substrate. To evaluate its potential, key components of the fermentation medium affecting GABA synthesis were re-screened and re-optimized to enhance GABA production from L. brevis NCL912. The initial fermentation medium (pH 3.3) used for optimization was: 50 g/L glucose, 25 g/L yeast extract, 10 mg/L manganese sulfate (MnSO4·H2O), 2 g/L Tween-80, and 220 g/L L-glutamic acid. Glucose, a nitrogen source, magnesium, and Tween-80 had notable effects on GABA production from the L-glutamic acid-based process; other factors showed no or marginal effects. The optimized levels of the four key components in the fermentation medium were 25 g/L glucose, 25 g/L yeast extract FM408, 25 mg/L MnSO4·H2O, and 2 g/L Tween-80. A simple and efficient fermentation process for the bioconversion of GABA by L. brevis NCL912 was ...Continue Reading

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Citations

Nov 26, 2019·Journal of the American College of Nutrition·Monika YadavPratyoosh Shukla
Feb 8, 2020·International Journal of Molecular Sciences·Yanhua CuiXiaojun Qu
Jan 13, 2021·Microorganisms·Valérie LarouteMuriel Cocaign-Bousquet
May 26, 2021·Bioprocess and Biosystems Engineering·Na Yeon KimChae Hun Ra

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