Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii

Plant Molecular Biology
Mark RütgersMichael Schroda

Abstract

We have identified 39 proteins that interact directly or indirectly with high confidence with chloroplast HSP22E/F under heat stress thus revealing chloroplast processes affected by heat. Under conditions promoting protein unfolding, small heat shock proteins (sHsps) prevent the irreversible aggregation of unfolding proteins by integrating into forming aggregates. Aggregates containing sHsps facilitate the access of Hsp70 and ClpB/Hsp104 chaperones, which in ATP-dependent reactions disentangle individual proteins from the aggregates and assist in their refolding to the native state. Chlamydomonas reinhardtii encodes eight different sHsps (HSP22A to H). The goal of this work was to identify chloroplast-targeted sHsps in Chlamydomonas and to obtain a comprehensive list of the substrates with which they interact during heat stress in order to understand which chloroplast processes are disturbed under heat stress. We show that HSP22E and HSP22F are major chloroplast-targeted sHsps that have emerged from a recent gene duplication event resulting from the ongoing diversification of sHsps in the Volvocales. HSP22E/F strongly accumulate during heat stress and form high molecular mass complexes. Using differential immunoprecipitation, m...Continue Reading

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Citations

Apr 17, 2020·The New Phytologist·Elizabeth R Waters, Elizabeth Vierling
Apr 22, 2020·Cell Stress & Chaperones·Afrooz Dabbaghizadeh, Robert M Tanguay
Oct 13, 2020·Journal of Phycology·David Carrasco FloresMaria Mittag
Dec 15, 2020·Plant, Cell & Environment·Scott HayesSteven A Arisz
Apr 25, 2021·Journal of Experimental Botany·Indu SanthanagopalanHoward Griffiths

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Datasets Mentioned

BETA
BP092687
AV642726
AV639812
AV642602

Methods Mentioned

BETA
PCR
immunoprecipitations
immunoprecipitation
electrophoresis
RNA-seq

Software Mentioned

MaxQuant
Microsoft #
ChloroP
Phylogeny
FSharp
Ploty
FUSIONCapt Advance
fr

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