Subtle Differences in Symbiont Cell Surface Glycan Profiles Do Not Explain Species-Specific Colonization Rates in a Model Cnidarian-Algal Symbiosis

Frontiers in Microbiology
John E ParkinsonVirginia M Weis

Abstract

Mutualisms between cnidarian hosts and dinoflagellate endosymbionts are foundational to coral reef ecosystems. These symbioses are often re-established every generation with high specificity, but gaps remain in our understanding of the cellular mechanisms that control symbiont recognition and uptake dynamics. Here, we tested whether differences in glycan profiles among different symbiont species account for the different rates at which they initially colonize aposymbiotic polyps of the model sea anemone Aiptasia (Exaiptasia pallida). First, we used a lectin array to characterize the glycan profiles of colonizing Symbiodinium minutum (ITS2 type B1) and noncolonizing Symbiodinium pilosum (ITS2 type A2), finding subtle differences in the binding of lectins Euonymus europaeus lectin (EEL) and Urtica dioica agglutinin lectin (UDA) that distinguish between high-mannoside and hybrid-type protein linked glycans. Next, we enzymatically cleaved glycans from the surfaces of S. minutum cultures and followed their recovery using flow cytometry, establishing a 48-72 h glycan turnover rate for this species. Finally, we exposed aposymbiotic host polyps to cultured S. minutum cells masked by EEL or UDA lectins for 48 h, then measured cell densi...Continue Reading

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Citations

Jun 1, 2019·Integrative and Comparative Biology·Virginia M Weis
Nov 7, 2019·Global Change Biology·Homère J Alves MonteiroJérémy Le Luyer
Oct 13, 2020·Trends in Microbiology·Sabrina L RossetSimon K Davy
Jun 15, 2021·Frontiers in Microbiology·Yu FujiwaraJohn Everett Parkinson

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Methods Mentioned

BETA
fluorescence microscopy
glycosylation
flow cytometry
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Software Mentioned

BayesFactor
R
stats
R Statistical Environment
LIMMA
ImageJ

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