Subtoxic Doses of Cadmium Modulate Inflammatory Properties of Murine RAW 264.7 Macrophages

BioMed Research International
Sina RiemschneiderJörg Lehmann

Abstract

Cadmium (Cd) is a toxic heavy metal that exhibits various adverse effects in the human and animal organism. Its resemblance to essential metals such as calcium, iron, and zinc leads to an unintended uptake in cells after intake through inhalation and ingestion. In this study we investigated the toxicity and the immunomodulatory potential of Cd in nonactivated and activated murine macrophages (i.e., cell line RAW 264.7). Cadmium alone caused a dose-dependent decreased viability of exposed cells. Subtoxic Cd concentrations delayed cell death in macrophages, resulting from cytotoxic storm, producing reactive oxygen species (ROS) and nitric oxide (NO), in response to their stimulation by bacterial antigens via pattern-recognition receptors (PRRs). In addition, production of selected pro- and anti-inflammatory cytokines, the chemokine CXCL1 (KC), and NO was determined. We observed that proinflammatory IL-1β and also CXCL1 were highly upregulated whereas anti-inflammatory or regulatory cytokines IL-6 and IL-10 were suppressed by 10 µM Cd. Also production of antibacterial NO was significantly reduced through exposure to 10 µM Cd, maybe explaining better survival of macrophages. Additionally, we could show by analysis via ICP-MS that d...Continue Reading

References

Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jun 1, 1997·Toxicology and Applied Pharmacology·D Beyersmann, S Hechtenberg
Mar 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Z KrocovaL Hernychova
Mar 18, 2003·Nihon eiseigaku zasshi. Japanese journal of hygiene·Masahiko SatohChiharu Tohyama
Mar 29, 2003·Toxicology and Applied Pharmacology·Masato OkuboHitoshi Endou
Jul 15, 2003·American Journal of Physiology. Endocrinology and Metabolism·Adam SteensbergBente Klarlund Pedersen
Apr 13, 2004·Journal of Environmental Quality·Karin WegglerRobin D Graham
Apr 24, 2004·Annals of the New York Academy of Sciences·Joseph P BresslerDesmond Bannona
Jul 9, 2004·Environmental Health Perspectives·Soisungwan Satarug, Michael R Moore
Jun 11, 2005·Cancer Investigation·Abe E SahmounGary G Schwartz
Mar 3, 2006·Toxicology·Nasr Y A HemdanIrina Lehmann
Mar 29, 2006·Clinics in Laboratory Medicine·Danyal IbrahimDaniel E Rusyniak
May 9, 2006·Toxicology and Applied Pharmacology·Constantinos GiaginisStamatios Theocharis
Nov 27, 2007·Biochemical and Biophysical Research Communications·Zhiwei LiuDaniel W Nebert
Feb 14, 2008·Molecular Pharmacology·Kuppuswami GirijashankerDaniel W Nebert
Mar 28, 2008·Reproductive Toxicology·Jennifer Thompson, John Bannigan
May 23, 2008·Archives of Toxicology·Detmar Beyersmann, Andrea Hartwig
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ceri A FieldingBrendan J Jenkins
Mar 8, 2011·Cell·Nicholas ArpaiaGregory M Barton
Aug 16, 2011·Metallomics : Integrated Biometal Science·Marisa Freitas, Eduarda Fernandes
Nov 19, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Estelle Cormet-BoyakaProsper N Boyaka

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Citations

Mar 5, 2019·Journal of Applied Toxicology : JAT·Diego García-MendozaNico W van den Brink
Mar 7, 2017·Frontiers in Endocrinology·Justin T SmithErin L Hsu
Jan 29, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ivana MirkovMilena Kataranovski
Aug 7, 2021·Frontiers in Immunology·Zhineng WangHui Wang
Dec 8, 2021·Toxicology and Industrial Health·Taru GoyalPraveen Sharma

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
ELISA
Assay

Software Mentioned

SigmaPlot
xCELLigence
Magellan
xCELLigence RTCA

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