Subtype distribution of Blastocystis in Thai-Myanmar border, Thailand

The Korean Journal of Parasitology
Supaluk PoprukAttakorn Palasuwan

Abstract

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevale...Continue Reading

Citations

Sep 19, 2015·Parasites & Vectors·Marawan Abu-MadiHanan Balkhy
Sep 24, 2016·The Korean Journal of Parasitology·Attakorn PalasuwanSupaluk Popruk
Dec 13, 2017·BMC Microbiology·Joakim ForsellMargareta Granlund
Oct 5, 2017·Journal of Tropical Pediatrics·Ika P SariAgnes Kurniawan
May 24, 2020·Transboundary and Emerging Diseases·Lei MaYinghui Guo
Dec 3, 2020·International Journal of Environmental Research and Public Health·Noppon PoprukDuangdao Palasuwan
Nov 3, 2021·BMC Infectious Diseases·Danuta Kosik-BogackaElżbieta Grochans

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Blast
MEGE

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