Subtype-specific interactions of type C staphylococcal enterotoxins with the T-cell receptor

Molecular Microbiology
J R DeringerG A Bohach

Abstract

The goal of this study was to investigate the molecular interaction between superantigens and the T-cell receptor (TCR). Using a quantitative polymerase chain reaction (PCR) to assess T-cell proliferation profiles, we found that SEB, SEC1, SEC2 and SEC3 expanded human T cells bearing V beta 3, V beta 12, V beta 13.2, V beta 14, V beta 15, V beta 17 and V beta 20. SEC2 and SEC3 have the additional ability to expand T cells bearing V beta 13.1, and their expansion of V beta 3 was markedly reduced compared to SEB and SEC1. Based on the activity of SEC1 mutants containing single amino acid substitutions, we concluded that the differential abilities of these native toxins to stimulate V beta 3 and V beta 13.1 was determined by the residue in position 26, located in the base of the SEC alpha 3 cavity. The SEC1 mutant, in which Val in position 26 was substituted with the analogous SEC2/SEC3 residue (Tyr), generated a V beta expansion profile that was indistinguishable from those generated by SEC2 and SEC3. Using these findings, the co-ordinates of a recently reported murine TCR beta-chain crystal structure, and other documented information, we propose a compatible molecular model for the interaction of SEC3 with the T-cell receptor. I...Continue Reading

Citations

Aug 6, 2000·The New England Journal of Medicine·D S Binder
Dec 19, 2000·Infection and Immunity·P M OrwinP M Schlievert
Feb 15, 2011·Journal of Bacteriology·Jyoti MadhusoodananSteven R Gill
Nov 6, 2015·Applied Microbiology and Biotechnology·Guojun ZhangChenggang Zhang
May 20, 2004·International Journal of Medical Microbiology : IJMM·Matthew D Baker, K Ravi Acharya
Mar 15, 2003·International Journal of Medical Microbiology : IJMM·Joseph E Alouf, Heide Müller-Alouf
Apr 13, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F L NowrouzianE Josefsson
Jul 31, 2007·Molecular Immunology·Erica LantelmeClaudia Giachino
Apr 13, 2017·Aesthetic Surgery Journal·Marshall E KadinCaroline A Glicksman
Jul 28, 2006·Journal of Veterinary Science·Yong Ho ParkGregory A Bohach
Sep 12, 2018·Infection and Immunity·Gillian J WilsonJ Ross Fitzgerald
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah C BangsXiao-Ning Xu
Feb 7, 1998·Infection and Immunity·W A FerensG Bohach
Jul 1, 1997·Infection and Immunity·M RoggianiP M Schlievert

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