Subtyping novel zoonotic pathogen Cryptosporidium chipmunk genotype I

Journal of Clinical Microbiology
Yaqiong GuoLihua Xiao

Abstract

Cryptosporidium chipmunk genotype I is an emerging zoonotic pathogen in humans. The lack of subtyping tools makes it impossible to determine the role of zoonotic transmission in epidemiology. To identify potential subtyping markers, we sequenced the genome of a human chipmunk genotype I isolate. Altogether, 9,509,783 bp of assembled sequences in 853 contigs were obtained, with an N50 of 117,886 bp and >200-fold coverage. Based on the whole-genome sequence data, two genetic markers encoding the 60-kDa glycoprotein (gp60) and a mucin protein (ortholog of cgd1_470) were selected for the development of a subtyping tool. The tool was used for characterizing chipmunk genotype I in 25 human specimens from four U.S. states and Sweden, one specimen each from an eastern gray squirrel, a chipmunk, and a deer mouse, and 4 water samples from New York. At the gp60 locus, although different subtypes were seen among the animals, water, and humans, the 15 subtypes identified differed mostly in the numbers of trinucleotide repeats (TCA, TCG, or TCT) in the serine repeat region, with only two single nucleotide polymorphisms in the nonrepeat region. Some geographic differences were found in the subtype distribution of chipmunk genotype I from huma...Continue Reading

References

Dec 5, 2002·International Journal for Parasitology·Lihua XiaoAltaf A Lal
Aug 9, 2005·Applied and Environmental Microbiology·Jianlin JiangLihua Xiao
Sep 29, 2006·Journal of Clinical Microbiology·Dawn C FeltusJohn M McEvoy
Aug 28, 2007·Applied and Environmental Microbiology·Yaoyu FengLihua Xiao
Nov 26, 2008·Experimental Parasitology·Michelle L PowerSally Williamson
Jan 27, 2009·Eukaryotic Cell·Jane W WanyiriHonorine D Ward
Apr 11, 2009·Experimental Parasitology·Lihua Xiao
May 1, 2009·Emerging Infectious Diseases·Rachel M ChalmersCatherine Mallaghan
Aug 10, 2010·Experimental Parasitology·Yaoyu FengLihua Xiao
Aug 11, 2012·Epidemiology and Infection·M InsulanderB Svenungsson
Jun 26, 2013·International Journal for Parasitology·Martin KváčJaroslav Piálek
Jan 23, 2014·Emerging Infectious Diseases·Na LiYaoyu Feng
Apr 18, 2014·Journal of Clinical Microbiology·Christen Rune StensvoldMarianne Lebbad

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Citations

Oct 6, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Brianna L S StengerJohn M McEvoy
Jun 7, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Fuchun JianLihua Xiao
Jun 21, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Anson V KoehlerRobin B Gasser
Jul 28, 2016·Parasite Immunology·U RyanA Paparini
Dec 23, 2016·Journal of Clinical Microbiology·Per SikoraRomanico B G Arrighi
Apr 3, 2015·Journal of Clinical Microbiology·C R StensvoldM Lebbad
Jun 18, 2017·Folia Parasitologica·Abd Elkarim LaatamnaMartin Kvac
Jan 11, 2020·Frontiers in Public Health·Arthur MorrisRachel M Chalmers
Sep 22, 2017·Frontiers in Microbiology·Yaoyu Feng, Lihua Xiao
Jan 25, 2020·Parasites & Vectors·Laura Rojas-LopezKarin Troell
Dec 11, 2020·Journal of Clinical Microbiology·Wen JiangLihua Xiao
Feb 26, 2021·Clinical Microbiology Reviews·Xin YangYaoyu Feng
Apr 14, 2021·International Journal for Parasitology·Jiayu LiLihua Xiao
May 11, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ioana BujilaJessica Beser

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