PMID: 9180028May 5, 1997Paper

Subtyping of D20S85 STR alleles by single-strand conformation polymorphism (SSCP) analysis

Forensic Science International : Synergy
W WangY Tamaki

Abstract

During a population study of STR locus D20S85, we discovered two types of sequence variations by direct sequencing of the alleles: two transitions each of G to A and A to G occur in the 5' flanking region in the individuals possessing allele 6 and some of those possessing allele 7 [1]. Using single-strand conformation polymorphism (SSCP) analysis, we were able to distinguish two subtypes of allele 7 from each other. This analysis method enables rapid screening for STR alleles of the same length with different sequences, and should find application to other complex STR loci because of the practical advantage of simplicity in comparison to sequencing.

References

Jun 1, 1992·Human Molecular Genetics·T PhromchotikulM Litt
Mar 11, 1992·Nucleic Acids Research·Y M LoJ S Wainscoat
Nov 25, 1991·Nucleic Acids Research·M IizukaT Sekiya
Oct 1, 1990·Genomics·M OritaK Hayashi
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Jan 1, 1994·International Journal of Legal Medicine·A Möller, B Brinkmann
Jan 1, 1993·Human Molecular Genetics·D BrowneM Litt
Jan 1, 1995·International Journal of Legal Medicine·A MöllerB Brinkmann

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