PMID: 6179545Jun 4, 1982Paper

Subunit and primary structure of a mouse alpha-macroglobulin, a human alpha 2-macroglobulin homologue

Biochimica Et Biophysica Acta
N W Hudson, P H Koo


A mouse alpha-macroglobulin (AMG), a homologue of human alpha 2-macroglobulin (alpha 2 M), has been purified to homogeneity. In contrast to human and acute-phase rat alpha 2 M which contains subunits of about Mr 190 000, the mouse protein contains two major (Mr 163000 and 35000) and one minor (Mr 185000) subunits. Also unlike human alpha 2 M, which can be broken down into about 85000-dalton subunits when reacted with an endopeptidase, the native AMG is cleaved by trypsin into multiple components (Mr 86000, 63000, 61000 and 33000). Two-dimensional peptide map analysis of these various 125I-labeled subunit components reveals that the 185000- and 163000-dalton components are homologous proteins but only the 185000-dalton protein contains the 35000-dalton component. The 163000-dalton protein is cleaved by trypsin into 86000- and 63000-dalton components, and the 86-kDa component in turn can be broken down into 61000- and 33000-dalton fragments. Since the 35000-dalton component is serologically related to AMG but does not share any tryptic peptides with both the 163000- and 33000-dalton components, it is neither a copurified impurity nor a cleavage product of the major (163000-dalton) subunit. AMG, therefore, is composed of covalentl...Continue Reading


Dec 1, 1976·The Biochemical Journal·A H Gordon
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R P Swenson, J B Howard
Nov 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·B H BerneK L Knight
Jan 22, 1968·Biochimica Et Biophysica Acta·M SteinbuchV Martin
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Apr 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Ohlsson
Jun 29, 1971·Biochimica Et Biophysica Acta·N D GreeneW J Hubbard
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER


Feb 1, 1993·Protein Science : a Publication of the Protein Society·F Van LeuvenH van den Berghe
Jan 1, 1992·Electron Microscopy Reviews·E DelainF Van Leuven
May 29, 2013·Fish & Shellfish Immunology·Wen-Hsiao ChuangPing-Chung Liu
Oct 17, 2001·Developmental and Comparative Immunology·B G Magor, K E Magor
Mar 1, 1989·Journal of Neuroscience Research·P H Koo, R W Stach
Mar 1, 1988·The Journal of Primary Prevention·L D GilchristV Senechal
Jan 1, 1983·Annals of the New York Academy of Sciences·P H Koo

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
L ZardiL Santi
International Journal of Cancer. Journal International Du Cancer
S ZuckerD K Strickland
© 2021 Meta ULC. All rights reserved