Subunit composition of a bicomponent toxin: staphylococcal leukocidin forms an octameric transmembrane pore

Protein Science : a Publication of the Protein Society
George MilesH Bayley

Abstract

Staphylococcal leukocidin pores are formed by the obligatory interaction of two distinct polypeptides, one of class F and one of class S, making them unique in the family of beta-barrel pore-forming toxins (beta-PFTs). By contrast, other beta-PFTs form homo-oligomeric pores; for example, the staphylococcal alpha-hemolysin (alpha HL) pore is a homoheptamer. Here, we deduce the subunit composition of a leukocidin pore by two independent methods: gel shift electrophoresis and site-specific chemical modification during single-channel recording. Four LukF and four LukS subunits coassemble to form an octamer. This result in part explains properties of the leukocidin pore, such as its high conductance compared to the alpha HL pore. It is also pertinent to the mechanism of assembly of beta-PFT pores and suggests new possibilities for engineering these proteins.

References

Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Montal, P Mueller
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J E GouauxH Bayley
Jun 1, 1993·Current Opinion in Neurobiology·A Karlin
Aug 19, 1997·Biochemistry·L HeginbothamC Miller
Jun 1, 1997·Journal of Medical Microbiology·B KönigW König
Feb 21, 1998·Current Biology : CB·H Bayley
Jan 7, 1998·Protein Science : a Publication of the Protein Society·E GouauxL Song
Mar 26, 1998·Journal of Molecular Biology·D M CzajkowskyZ Shao
Feb 27, 1999·Current Opinion in Biotechnology·H Bayley
Jul 1, 1999·Protein Science : a Publication of the Protein Society·S CheleyH Bayley
Nov 27, 1999·Science·D StockJ E Walker
Jun 6, 2000·Nature·H SeelertD J Müller
Oct 6, 2000·Molecular Biology and Evolution·J M ArchibaldW F Doolittle
Mar 27, 2001·EMBO Reports·H StahlbergP Dimroth
Apr 12, 2001·Current Opinion in Structural Biology·S C Harrison
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·W JiangR H Fillingame
Sep 15, 2001·Nature·H Bayley, P S Cremer
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·G MenestrinaG Prévost

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Citations

Mar 27, 2010·The Journal of Membrane Biology·Beatrix Vécsey-SemjénRoland Möllby
Nov 1, 2011·Seminars in Immunopathology·Dane Parker, Alice Prince
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Michael W Parker, Susanne C Feil
Nov 5, 2013·Revue de pneumologie clinique·F ValourT Ferry
May 10, 2011·Biochemistry·Belete R ChenekeLiviu Movileanu
Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
Jun 15, 2010·The Journal of Physical Chemistry. B·Mohammad M Mohammad, Liviu Movileanu
Aug 15, 2009·Nature Reviews. Microbiology·Alan R Hauser
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Keitaro YamashitaIsao Tanaka
Feb 18, 2011·The Journal of Biological Chemistry·Anne F HammersteinHagan Bayley
Apr 15, 2011·The Journal of Biological Chemistry·Kengo KitadokoroYasuhiko Horiguchi
May 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel J Gordon, Franklin D Lowy
Dec 4, 2009·Current Opinion in Critical Care·Samuel M Moskowitz, Jeanine P Wiener-Kronish
Jun 19, 2002·Protein Science : a Publication of the Protein Society·George MilesStephen Cheley
Nov 10, 2009·Journal of Bacteriology·Takashige KashimotoNobuyuki Susa
May 15, 2007·Journal of Biomedicine & Biotechnology·Olivier JoubertGilles Prévost
Nov 16, 2013·Trends in Microbiology·Ashley L DuMont, Victor J Torres
Sep 17, 2004·Molecular Membrane Biology·Hagan Bayley, Lakmal Jayasinghe
Dec 15, 2015·Pathogens and Disease·Sergey M Bezrukov, Ekaterina M Nestorovich
Mar 26, 2010·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Tatsuo YamamotoDa Shi
Apr 28, 2009·Trends in Biotechnology·Liviu Movileanu
Oct 1, 2005·Protein Science : a Publication of the Protein Society·Lakmal Jayasinghe, Hagan Bayley
Sep 4, 2015·Nature Communications·Pauline Yoong, Victor J Torres
Aug 15, 2015·Nature Reviews. Microbiology·Vilasack ThammavongsaOlaf Schneewind
Jan 4, 2013·The Biochemical Journal·Benoît-Joseph LaventieGilles Prévost
Feb 27, 2008·Biochimica Et Biophysica Acta·Ioan IacovacheLucile Pernot
Feb 8, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V ChiniI Spiliopoulou

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