Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans

Proceedings of the National Academy of Sciences of the United States of America
Yushu ChenMartin Chalfie

Abstract

Caenorhabditis elegans senses gentle touch in the six touch receptor neurons (TRNs) using a mechanotransduction complex that contains the pore-forming degenerin/epithelial sodium channel (DEG/ENaC) proteins MEC-4 and MEC-10. Past work has suggested these proteins interact with the paraoxonase-like MEC-6 and the cholesterol-binding stomatin-like MEC-2 proteins. Using single molecule optical imaging in Xenopus oocytes, we found that MEC-4 forms homotrimers and MEC-4 and MEC-10 form 4:4:10 heterotrimers. MEC-6 and MEC-2 do not associate tightly with these trimers and do not influence trimer stoichiometry, indicating that they are not part of the core channel transduction complex. Consistent with the in vitro data, MEC-10, but not MEC-6, formed puncta in TRN neurites that colocalize with MEC-4 when MEC-4 is overexpressed in the TRNs.

References

Jun 3, 2017·PloS One·Stefanie RungaldierRainer Prohaska
Oct 21, 2016·American Journal of Physiology. Cell Physiology·Cristina MatthewmanLaura Bianchi
Apr 26, 2018·American Journal of Physiology. Cell Physiology·Cristina MatthewmanLaura Bianchi
Aug 5, 2017·The Journal of Biological Chemistry·Shujie ShiThomas R Kleyman
Feb 22, 2020·Annual Review of Neuroscience·Peng JinYuh-Nung Jan
Feb 5, 2020·The Journal of General Physiology·Robert C KlippJohn R Bankston
Jul 26, 2016·Biomedical Optics Express·Michael ShawMandayam A Srinivasan
May 18, 2016·The Journal of Biological Chemistry·Shujie ShiThomas R Kleyman
May 14, 2016·G3 : Genes - Genomes - Genetics·Yushu ChenMartin Chalfie

Citations

Mar 1, 1981·Developmental Biology·Martin Chalfie, J Sulston
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher J BensonPeter M Snyder
Mar 5, 2002·Nature·Miriam B GoodmanMartin Chalfie
Jul 10, 2003·Neuron·Lei LiuMichael J Welsh
Aug 28, 2003·Médecine sciences : M/S·Christelle Gally, Jean-Louis Bessereau
Jun 11, 2004·Biophysical Journal·Sylvain V CostesStephen Lockett
Dec 30, 2004·The Journal of General Physiology·Michael B ButterworthRaymond A Frizzell
Nov 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tobias B HuberThomas Benzing
Jan 31, 2007·The Journal of General Physiology·Austin L BrownMiriam B Goodman
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dattananda S Chelur, Martin Chalfie
Mar 21, 2007·Nature Methods·Maximilian H Ulbrich, Ehud Y Isacoff
Sep 21, 2007·Nature·Jayasankar JastiEric Gouaux
Dec 21, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juan G CuevaMiriam B Goodman
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maximilian H Ulbrich, Ehud Y Isacoff
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Martin Chalfie
Feb 23, 2010·Current Biology : CB·Lixian ZhongW Daniel Tracey
Oct 1, 2010·Journal of Neurophysiology·Marios ChatzigeorgiouWilliam R Schafer
Jan 12, 2011·Neuron·Liliane AbuinRichard Benton
Sep 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jóhanna ArnadóttirMartin Chalfie
Sep 29, 2011·Proteins·Michael A HicksPatricia C Babbitt
May 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tudor BartoiMaximilian H Ulbrich
Dec 3, 2014·Current Biology : CB·Stephanie E MauthnerW Daniel Tracey
Dec 3, 2014·Cell Reports·Yanmeng GuoZuoren Wang
Dec 5, 2014·Nature·Sanjeev S RanadeArdem Patapoutian

Related Concepts

Aryldialkylphosphatase
trimer core
MEC-10 protein, C elegans
Epithelial sodium channel
Meissner's Corpuscle
Paraoxonase-2
Caenorhabditis elegans
C-C Motif Chemokine 28
Xenopus Oocyte
MEC-6 protein, C elegans

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.