Subunit composition of AMPK trimers present in the cytokinetic apparatus: Implications for drug target identification.

Cell Cycle
Katalin PinterCharles Redwood

Abstract

AMP-activated protein kinase has been shown to be a key regulator of energy homeostasis; it has also been identified as a tumor suppressor and is required for correct cell division and chromosome segregation during mitosis. The enzyme is a heterotrimer, with each subunit having more than one isoform, each encoded by a separate gene (two α, two β and three γ isoforms). In human endothelial cells, the activated kinase subunit of AMPK in the cytokinetic apparatus is α2, the minority α subunit, which co-localizes with β2 and γ2. This is the first demonstration of a trimeric complex of AMPK containing the γ2 regulatory subunit becoming selectively activated and being linked to mitotic processes. We also show that α1 and γ1, the predominant AMPK subunits, are almost exclusively localized in the cytoskeleton, while α2 and γ2 are present in all subcellular fractions, including the nuclei. These data suggest that pharmacological interventions targeted to specific AMPK subunit isoforms have the potential to modify selective functions of AMPK.

References

Mar 29, 2003·Diabetes·Sean L McGeeMark Hargreaves
May 16, 2003·Current Biology : CB·Galina PolekhinaDavid Stapleton
Jan 15, 2004·The Journal of Clinical Investigation·John W ScottD Grahame Hardie
Feb 19, 2005·The Journal of Physiology·Jørgen F P WojtaszewskiFlemming Dela
Aug 2, 2006·Annual Review of Pharmacology and Toxicology·D Grahame Hardie
Oct 14, 2006·The Journal of Physiology·J B Birk, J F P Wojtaszewski
Feb 18, 2009·Cell Cycle·Alejandro Vazquez-MartinJavier A Menendez
Feb 28, 2009·Acta Physiologica·J S OakhillB E Kemp
May 16, 2009·The Biochemical Journal·Sergio L Colombo, Salvador Moncada
Aug 7, 2009·American Journal of Physiology. Cell Physiology·Jonas T TreebakJørgen F P Wojtaszewski
Oct 22, 2009·Cell Cycle·Alejandro Vazquez-MartinJavier A Menendez
Mar 2, 2010·Cell Cycle·Fulvio Chiacchiera, Cristiano Simone
Jul 10, 2010·International Journal of Radiation Oncology, Biology, Physics·Toran SanliTheodoros Tsakiridis
Aug 6, 2010·Molecular Biology of the Cell·Nevzat KazganJay E Brenman
Sep 17, 2010·American Journal of Physiology. Cell Physiology·Lindsey D Bogachus, Lorraine P Turcotte
Oct 1, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marc ForetzBenoit Viollet
Jul 20, 2011·The Journal of Cell Biology·Tz-Chuen JuYijuang Chern
Sep 14, 2011·Oncotarget·Takeshi NamikiVincent J Hearing

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Citations

Dec 24, 2014·Expert Opinion on Therapeutic Patents·Joungmok KimJoohun Ha
Jun 12, 2012·Journal of Molecular and Cellular Cardiology·Katalin PinterCharles Redwood
Dec 16, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rasmus KjøbstedLouise Lantier
Sep 17, 2013·Journal of Muscle Research and Cell Motility·Katalin PinterCharles Redwood
Oct 28, 2017·Circulation Research·Ye Ding, Ming-Hui Zou
Nov 5, 2013·The Journal of Biological Chemistry·Jiang WuKieran F Geoghegan
Mar 5, 2016·The FEBS Journal·Fiona A RossD Grahame Hardie

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