PMID: 2120056Sep 11, 1990Paper

Subunit constitution of carbonic anhydrase from Chlamydomonas reinhardtii

European Journal of Biochemistry
T KamoS Miyachi

Abstract

Carbonic anhydrase purified from the cell surface of Chlamydomonas reinhardtii was inactivated by treatment with dithiothreitol. This treatment caused dissociation of the holoenzyme into 35-kDa (A) and 4-kDa (B) subunits as revealed by SDS/PAGE. The 35-kDa subunit was further separated into two components A1 (35 kDa) and A2 (36.5 kDa) by SDS/PAGE using a gradient gel. These two components have the same amino acid sequence up to at least the 10th amino acid from the N-terminus. The molecular masses were estimated at 76 kDa and 35 kDa for the holoenzyme and the large subunit, respectively, and the molar ratio of the former to the latter at 1:2, by using the techniques of low-angle laser light-scattering photometry and precision differential refractometry combined with gel-filtration HPLC. The molar ratio of the 35-kDa/4-kDa subunits was estimated at 1:1 the gel-filtration HPLC monitored with precision differential refractometry. Atomic-absorption spectrophotometry revealed that the holoenzyme contains two atoms of zinc. These results suggest that the holoenzyme is a heterotetramer composed of two large subunits (A1 and A2) and two small subunits (B).

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H FukuzawaS Miyachi
Aug 1, 1986·European Journal of Biochemistry·T ToguriS Miyachi
Mar 22, 1985·Science·D J Lipman, W R Pearson
Jan 1, 1984·Annals of the New York Academy of Sciences·D Hewett-EmmettJ Czelusniak
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J R Coleman, A R Grossman
Oct 1, 1984·Plant Physiology·J R ColemanA R Grossman

❮ Previous
Next ❯

Citations

Nov 1, 1993·The International Journal of Biochemistry·T Tanaka, E Ichishima
Jan 1, 1997·Pharmacology & Therapeutics·S Lindskog
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S FujiwaraS Miyachi
Sep 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kaoru SuzukiShi Yuan Yang
Sep 9, 2000·FEMS Microbiology Reviews·K S Smith, J G Ferry
May 15, 1993·European Journal of Biochemistry·S IshidaS Miyachi
Nov 5, 2015·Biochemistry. Biokhimii︠a︡·N N RudenkoB N Ivanov
Jul 26, 1996·The Journal of Biological Chemistry·M FisherA Zamir
Oct 30, 2020·Protoplasma·Natalia N RudenkoMaria M Borisova-Mubarakshina
Apr 1, 1993·Phytochemistry·N YamamotoE Ichishima
Apr 1, 1992·Archives of Biochemistry and Biophysics·K Shimogawara, S Muto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.