Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex

Life Science Alliance
Melanie Korntner-VetterMartin R Singleton

Abstract

Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1CENP-A Cell cycle-specific deposition of Cnp1 requires the Mis16-Mis18-Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18-Mis19 interface.

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Citations

Mar 24, 2021·Seminars in Cell & Developmental Biology·Alexandra P Navarro, Iain M Cheeseman

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Methods Mentioned

BETA
pull-down
size-exclusion chromatography
gel filtration
co-purification
isothermal titration calorimetry
pull-downs
affinity purification
size-exclusion
competition binding
light scattering

Software Mentioned

MicroCal
DIALS
phenix
MALLS
Coot
Xia2
ORIGIN
SEDFIT

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