PMID: 11914134Mar 27, 2002Paper

Subunit modification and association in VR1 ion channels

BMC Neuroscience
Tamara RosenbaumSharona E Gordon

Abstract

The capsaicin (vanilloid) receptor, VR1, is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal noxious stimuli. In the present study we investigated the properties of VR1 ion channels expressed in Xenopus oocytes. A VR1 subunit with a FLAG epitope tag at the C-terminus was constructed. When examined for size on an SDS gel, VR1-expressing oocytes produced a doublet corresponding to the size of the monomer and a band at about twice the molecular weight of the monomer. A consensus site for N-linked glycosylation was identified in the primary sequence at position 604. In channels in which the putative glycosylation site was mutated from asparagine to serine (N604S), the larger of the two monomer bands could no longer be detected on the gel. Electrophysiological experiments showed these unglycosylated channels to be functional. The high molecular weight band observed on the gel could represent either a dimer or a monomer conjugated to an unknown factor. To distinguish between these possibilities, we coexpressed a truncated VR1 subunit with full-length VR1. A band of intermediate molecular weight (composed of one full-length and one truncated subunit) was observed. This dim...Continue Reading

Citations

Mar 31, 2004·British Journal of Pharmacology·Pierangelo Geppetti, Marcello Trevisani
May 3, 2012·Lab on a Chip·Ahmad M El-ArabiJacob J Schmidt
Feb 26, 2004·The Journal of General Physiology·Tamara RosenbaumSharona E Gordon
Oct 4, 2006·The Journal of Biological Chemistry·Ilaria DragoniPeter McIntyre
Sep 26, 2009·The Journal of Biological Chemistry·Sonia Gallego-SandínJavier García-Sancho
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Miguel Ortíz-RenteríaSara L Morales-Lázaro
May 17, 2008·The Journal of Biological Chemistry·Irina VetterPeter J Cabot
Dec 31, 2003·The Journal of General Physiology·Tamara RosenbaumSharona E Gordon
Sep 2, 2009·The Journal of General Physiology·Beiying LiuFeng Qin
Oct 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sebastian BrauchiRamon Latorre
Aug 16, 2005·British Journal of Pharmacology·Kathy G SuttonGuy R Seabrook
Oct 31, 2012·The Journal of General Physiology·Sze-Yi LauRachelle Gaudet
Jan 18, 2005·Alternatives to Laboratory Animals : ATLA·Johanna Lilja, Anna Forsby
Jul 14, 2004·The Journal of Experimental Medicine·Alexander J StokesHelen Turner

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