PMID: 3289967Jun 6, 1988

Subunit-specific phosphorylation of pyruvate kinase in medullary thyroid carcinomas of the rat

FEBS Letters
G RijksenG E Staal

Abstract

Pyruvate kinase from anaplastic medullary thyroid carcinomas contains predominantly K-type subunits, whereas pyruvate kinase from differentiated medullary thyroid carcinomas consist of M- and K-type subunits in about equal proportion. In order to analyse the incorporation of phosphate in the respective isozymes after endogenous phosphorylation of cytosolic extracts with [32P]ATP, homotetrameric isozymes as well as heterotetrameric hybrids of differentiated tumors were resolved by affinity chromatography on Blue-Sepharose CL-6B and, if necessary, further purified by immunoprecipitation. SDS-polyacrylamide gel electrophoresis of purified isozymes and subsequent autoradiography showed the incorporation of phosphate in the K4-type isozymes, but not in the other isozymes. The phosphorylation appeared to be cAMP-independent and occurred on a serine residue.

References

Jul 1, 1996·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·F SchwägeleK O Honikel

Citations

Jul 1, 1981·Molecular and Cellular Endocrinology·H GlossmannE Eigenbrodt
Jan 1, 1983·Methods in Enzymology·J A CooperT Hunter
Sep 15, 1983·Biochemical and Biophysical Research Communications·P FisterW Schoner
Aug 6, 1982·Biochimica Et Biophysica Acta·B ScheringW Schoner

Related Concepts

Macromolecular Compounds
SDS-PAGE
Carcinomatosis
Neoplasms
Electrophoresis, Cellulose Acetate
Protein Multimerization
R-Type Pyruvate Kinase
Immunoassay Method
Orthophosphate
Medullary Carcinoma of Thyroid

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