PMID: 8997668Jan 1, 1997Paper

Subunit stoichiometry of human muscle chloride channels

The Journal of General Physiology
Christoph FahlkeA L George

Abstract

Voltage-gated Cl- channels belonging to the ClC family appear to function as homomultimers, but the number of subunits needed to form a functional channel is controversial. To determine subunit stoichiometry, we constructed dimeric human skeletal muscle Cl- channels in which one subunit was tagged by a mutation (D136G) that causes profound changes in voltage-dependent gating. Sucrose-density gradient centrifugation experiments indicate that both monomeric and dimeric hClC-1 channels in their native configurations exhibit similar sedimentation properties consistent with a multimeric complex having a molecular mass of a dimer. Expression of the heterodimeric channel in a mammalian cell line results in a homogenous population of Cl- channels exhibiting novel gating properties that are best explained by the formation of heteromultimeric channels with an even number of subunits. Heteromultimeric channels were not evident in cells cotransfected with homodimeric WT-WT and D136G-D136G constructs excluding the possibility that functional hClC-1 channels are assembled from more than two subunits. These results demonstrate that the functional hClC-1 unit consists of two subunits.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C K BauerT J Jentsch
Dec 1, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Miller
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 1, 1983·The Journal of General Physiology·W Hanke, C Miller
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C Miller, M M White
Apr 21, 1995·The Journal of Biological Chemistry·C A GurnettK P Campbell
Jan 1, 1995·The American Journal of Physiology·D H MalinowskaJ Cuppoletti
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KieferleT J Jentsch
Apr 1, 1994·Human Molecular Genetics·M A van SlegtenhorstA Ballabio
Aug 1, 1996·Biophysical Journal·C FahlkeR Rüdel

❮ Previous
Next ❯

Citations

Sep 5, 2002·Muscle & Nerve·Kleopas A Kleopa, Robert L Barchi
May 25, 2011·Pflügers Archiv : European journal of physiology·Karl KunzelmannRainer Schreiber
Nov 17, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·I Elias Veizis, Calvin U Cotton
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Apr 30, 1998·Molecular Aspects of Medicine·B FontaineS Nicole
Aug 7, 2002·Current Opinion in Structural Biology·Raúl Estévez, Thomas J Jentsch
Oct 27, 2010·Molecular & Cellular Proteomics : MCP·Ghada FallahGünther Schmalzing
Nov 14, 1997·The Journal of General Physiology·C FahlkeA L George
Aug 30, 2000·The Journal of General Physiology·A Accardi, M Pusch
Jun 29, 2001·The Journal of General Physiology·M F Chen, T Y Chen
Apr 29, 1998·Annual Review of Physiology·J K Foskett
Apr 18, 2001·American Journal of Respiratory Cell and Molecular Biology·F S LambP B McCray
Sep 9, 2008·Journal of the American Society of Nephrology : JASN·Audrey G H JanssenChristoph Fahlke
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C FahlkeA L George
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·T Schmidt-Rose, T J Jentsch
Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ute SchollChristoph Fahlke
Nov 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hiroaki MiyazakiYoshio Takei
Mar 19, 2014·Pflügers Archiv : European journal of physiology·Gabriel StöltingChristoph Fahlke
Feb 19, 2000·Annals of Medicine·N P Davies, M G Hanna
Mar 8, 2002·The Journal of Physiology·Carlos G Vanoye, Alfred L George
Jan 16, 2004·The Journal of Physiology·Leandro ZúñigaFrancisco V Sepúlveda
Sep 20, 2008·The Journal of Physiology·Jennie Garcia-OlivaresChristoph Fahlke
Apr 30, 2004·Muscle & Nerve·Lie ChenJean-Marc Burgunder
Apr 25, 2006·Acta Neurologica Scandinavica·H-C KuoC-C Huang
Jan 12, 2002·The Veterinary Clinics of North America. Small Animal Practice·Charles H Vite
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M MadukeJ A Mindell
Mar 2, 1999·The Journal of Physiology·M PuschT J Jentsch
Jan 12, 1999·The Journal of Physiology·A RosenbohmC Fahlke
Jan 14, 2004·The Journal of Biological Chemistry·Simon HebeisenChristoph Fahlke
Oct 4, 2017·The Journal of Biological Chemistry·Raul E GuzmanChristoph Fahlke
Aug 26, 1998·Nature·C FahlkeA L George
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
Nov 30, 2000·Current Treatment Options in Neurology·I I I Moxley
Jun 6, 2006·Journal of Biochemistry·Shu Jie LiAtsushi Nakagawa
Oct 18, 2000·The Journal of Biological Chemistry·C FahlkeA L George

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.