PMID: 2496750Jan 24, 1989Paper

Subunit structure of thrombin-activated porcine factor VIII

Biochemistry
P Lollar, C G Parker

Abstract

Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) derived from the B domain being difficult to visualize. The appearance of these fragments is associated with the development of coagulant activity, but the activity is labile without further apparent proteolysis. In this study, porcine fVIII was reacted with thrombin until peak coagulant activity was obtained and then subjected to cation-exchange (Mono S) high-pressure liquid chromatography. Coagulant activity was recovered in a single peak that contained all three fragments and was stable for weeks at 20 degrees C in 0.65 M NaCl/0.01 M His-HCl/0.005 M CaCl2 at pH 6.0. Analytical ultracentrifugation of activated fVIII was done to test whether all three fragments were associated. The apparent molecular weight of activated fVIII from equilibrium sedimentation increased from 148,000 to 161,000 as the loading concentration was increased from 0.06 to 0.16 mg/mL. This agrees well with the summed apparent molecular weights...Continue Reading

References

Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Sep 1, 1975·The Journal of Clinical Investigation·H A CooperR H Wagner
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L O AnderssonJ Smart
Jun 23, 1986·Biochimica Et Biophysica Acta·P J FayV J Marder
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W H Kane, E W Davie
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J JennyK G Mann
Sep 1, 1987·European Journal of Biochemistry·R J HamerJ J Sixma
Jan 29, 1988·Biochimica Et Biophysica Acta·P J Fay
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D D Pittman, R J Kaufman
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D N FassK G Mann
Dec 31, 1985·Biochemistry·P LollarD N Fass
Sep 1, 1984·Archives of Biochemistry and Biophysics·P Lollar, D N Fass
Nov 22, 1984·Nature·J GitschierR M Lawn
Nov 22, 1984·Nature·G A VeharD J Capon
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A Fulcher, T S Zimmerman
Mar 1, 1964·Biochemistry·D A YPHANTIS

❮ Previous
Next ❯

Citations

Jan 28, 2009·Clinical Reviews in Allergy & Immunology·Géraldine Lavigne-LissaldeClaude Granier
Nov 1, 1995·Thrombosis Research·F ManningB Woodhams
Jan 1, 1993·Biotechnology Advances·F ManningR O'Kennedy
Dec 20, 2003·Blood Reviews·Philip J Fay
Jan 30, 2003·Trends in Cardiovascular Medicine·Amy E Schmidt, S Paul Bajaj
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·S S AhmadP N Walsh
Jan 6, 1994·The New England Journal of Medicine·L W Hoyer
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D C Hill-EubanksP Lollar
Oct 6, 2009·The Journal of Biological Chemistry·Indu JagannathanPhilip J Fay
Sep 25, 1999·Human Gene Therapy·R J Kaufman
Sep 1, 1990·British Journal of Haematology·G Kemball-CookT W Barrowcliffe
Jun 1, 1991·British Journal of Haematology·G Kemball-CookT W Barrowcliffe
Jun 1, 1994·The Journal of Clinical Investigation·P LollarD Scandella
Nov 12, 2013·Journal of Thrombosis and Thrombolysis·Kathleen E Brummel-ZiedinsThomas Orfeo
Jan 1, 1991·Annals of the New York Academy of Sciences·D N Fass
Jan 1, 1991·Annals of the New York Academy of Sciences·K G MannS Krishnaswamy
May 1, 1991·Mayo Clinic Proceedings·P Lollar
Jan 16, 2015·Thrombosis and Haemostasis·Kirill GrushinSvetla Stoilova-McPhie
Jun 29, 2004·Journal of Thrombosis and Haemostasis : JTH·P Lollar
Feb 4, 2009·Journal of Thrombosis and Haemostasis : JTH·S L MeeksP Lollar
Apr 23, 2014·Haemophilia : the Official Journal of the World Federation of Hemophilia·K GrushinS Stoilova-McPhie
Sep 29, 2004·Biochimica Et Biophysica Acta·Keiji NogamiPhilip J Fay
Oct 1, 1989·Baillière's Clinical Haematology·E G Tuddenham
Jun 18, 2015·Scientific Reports·Daniela DalmSvetla Stoilova-McPhie
Nov 26, 2015·Scientific Reports·Michelle E WuerthP Clint Spiegel
Mar 17, 1992·Biochemistry·D P O'BrienE G Tuddenham
Dec 9, 2003·The Journal of Biological Chemistry·Christopher B DoeringPete Lollar
Aug 30, 1996·The Journal of Biological Chemistry·J VoorbergM J Donath
Feb 14, 1991·European Journal of Biochemistry·S KrishnanC Roitsch
Mar 4, 2006·The Journal of Biological Chemistry·Ernest T ParkerPete Lollar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D D Pittman, R J Kaufman
Proceedings of the National Academy of Sciences of the United States of America
D C Hill-EubanksP Lollar
© 2022 Meta ULC. All rights reserved