Subunit swapping in the Mex-extrusion pumps in Pseudomonas aeruginosa

Biochemical and Biophysical Research Communications
H YoneyamaTaiji Nakae

Abstract

Pseudomonas aeruginosa encodes three sets of antibiotic extrusion proteins designated as MexA,B.OprM, MexC,D-OprJ and MexE,F-OprN regulated by the nalB, nfxB and nfxC genes, respectively. MexB,D,F, OprM, J,N and MexA,C,E function as the inner membrane pumps, the outer membrane channels and the membrane fusion proteins, respectively. To investigate the possibility of subunit interchangeability, we constructed the following combinations of chimeric pumps: MexA,D-OprM/delta MexB, MexC,B-OprM/delta MexA, and MexA,B-OprJ/delta OprM. The strains producing MexA,D-OprM/delta MexB and MexC,B-OprM/delta MexA failed to restore the antibiotic resistance shown in the strains producing the natural combinations of the subunit proteins. These results suggested that the inner membrane components cannot be interchanged. In contrast, the stains producing MexA,B-OprJ/delta OprM exhibited higher resistance to several antibiotics than the mutant lacking OprM and lower resistance than the strain overexpressing OprM. This result suggests that OprJ may complement the OprM function partially. A spectrum of antibiotics, of which the minimum inhibitory concentrations were restored partially by the complementation, was the same as the spectrum to which the...Continue Reading

References

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Citations

Aug 10, 2000·Molecular Microbiology·H I Zgurskaya, H Nikaido
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·N MasudaT Nishino
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·James T H JoRobert E W Hancock
Nov 12, 2002·Journal of Bacteriology·Elena B TikhonovaHelen I Zgurskaya
Oct 2, 2002·European Journal of Biochemistry·Eisaku YoshiharaKohjiro Saito
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Jun 28, 2003·FEMS Microbiology Reviews·Christopher Rensing, Gregor Grass
Aug 25, 2004·Biochemical and Biophysical Research Communications·Vladislav V MokhonovTaiji Nakae
May 6, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kazuki SatoHiroshi Yoneyama
Jul 1, 2009·Microbial Biotechnology·Ana SeguraGail M Preston
Jan 14, 2004·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Jan 24, 2007·Microbiology and Immunology·Eisaku Yoshihara, Shima Eda
Nov 27, 2007·Microbiology and Immunology·M Mushfequr RahmanTomofusa Tsuchiya
Mar 14, 2007·The Journal of Biological Chemistry·Shin-Ichiro Narita, Hajime Tokuda
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·David C. Hooper

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