PMID: 9421522Feb 28, 1998Paper

Subunits of human replication protein A are crosslinked by photoreactive primers synthesized by DNA polymerases

Nucleic Acids Research
O I LavrikA Favre

Abstract

Human replication protein A (huRPA) is a multisubunit protein which is involved in DNA replication, repair and recombination processes. It exists as a stable heterotrimer consisting of p70, p32 and p14 subunits. To understand the contribution of huRPA subunits to DNA binding we applied the photoaffinity labeling technique. The photoreactive oligonucleotide was synthesized in situ by DNA polymerases. 5-[N-(2-nitro-5-azidobenzoyl)-trans -3-aminopropenyl-1]deoxyuridine-5'-triphosphate (NABdUTP) was used as substrate for elongation of a radiolabeled primer logical ortemplate either by human DNA polymerase alpha primase (polalpha), human DNA polymerase beta (polbeta) or Klenow fragment of Escherichia coli DNA polymerase I (KF). The polymerase was incubated with NABdUTP and radiolabeled primer-template in the presence or absence of huRPA. The reaction mixtures were then irradiated with monochromatic UV light (315 nm) and the crosslinked products were separated by SDS-PAGE. The results clearly demonstrate crosslinking of the huRPA p70 and p32 subunits with DNA. The p70 subunit appears to bind to the single-stranded part of the DNA duplex, the p32 subunit locates near the 3'-end of the primer, while the p14 subunit locates relatively f...Continue Reading

References

Aug 24, 1992·FEBS Letters·A GeorgakiU Hübscher
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·A DuttaB Stillman
Sep 1, 1989·Molecular and Cellular Biology·T TsurimotoB Stillman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S Wold, T Kelly
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F B DeanJ Hurwitz
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M S WoldT J Kelly
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C M Joyce, N D Grindley
Mar 3, 1995·The Journal of Biological Chemistry·X V Gomes, M S Wold
Jun 15, 1994·European Journal of Biochemistry·F StadlbauerH P Nasheuer
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·E StiggerS H Lee
May 31, 1996·Journal of Molecular Biology·K TreunerR Knippers
Sep 1, 1996·Genes & Development·D PhilipovaS J Brill

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Citations

Jan 5, 2001·Nucleic Acids Research·D M KolpashchikovO I Lavrik
Feb 27, 2009·Nucleic Acids Research·Anne M DicksonMarc S Wold
Oct 9, 2012·Nucleic Acids Research·Rajendra PrasadSamuel H Wilson
Jul 26, 2002·Molecular and Cellular Biology·Joyce T Reardon, Aziz Sancar
Feb 21, 2007·Biochemistry. Biokhimii︠a︡·E L Vodovozova
May 17, 2011·Biochemistry. Biokhimii︠a︡·N I RechkunovaO I Lavrik
Aug 14, 2001·Journal of Molecular Recognition : JMR·D M KolpashchikovO I Lavrik
Jun 19, 2004·The Journal of Biological Chemistry·Klaus WeisshartHeinz-Peter Nasheuer
Mar 30, 2001·The Journal of Biological Chemistry·G MagaU Hubscher
Sep 9, 2010·Bioorganicheskaia khimiia·A V UstinovV A Korshun
Feb 6, 2020·Photochemistry and Photobiology·Nadejda I Rechkunova, Olga I Lavrik
Aug 17, 2004·The Journal of Biological Chemistry·Matthew J DellaVecchiaBennett Van Houten
Jan 8, 2000·Journal of Inorganic Biochemistry·J J TurchiS M Patrick

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