Successful cloning of a superior buffalo bull

Scientific Reports
Naresh L SelokarPrem Singh Yadav

Abstract

Somatic cell nuclear transfer (SCNT) technology provides an opportunity to multiply superior animals that could speed up dissemination of favorable genes into the population. In the present study, we attempted to reproduce a superior breeding bull of Murrah buffalo, the best dairy breed of buffalo, using donor cells that were established from tail-skin biopsy and seminal plasma. We studied several parameters such as cell cycle stages, histone modifications (H3K9ac and H3K27me3) and expression of developmental genes in donor cells to determine their SCNT reprogramming potentials. We successfully produced the cloned bull from an embryo that was produced from the skin-derived cell. Growth, blood hematology, plasma biochemistries, and reproductive organs of the produced cloned bull were found normal. Subsequently, the bull was employed for semen production. Semen parameters such as CASA (Computer Assisted Semen Analysis) variables and in vitro fertilizing ability of sperms of the cloned bull were found similar to non-cloned bulls, including the donor bull. At present, we have 12 live healthy progenies that were produced using artificial insemination of frozen semen of the cloned bull, which indicate that the cloned bull is fertile ...Continue Reading

References

Jun 14, 2000·Animal Reproduction Science·B Larsson, H Rodríguez-Martínez
Jul 23, 2004·Cloning and Stem Cells·Y HeymanC Galli
Jul 23, 2004·Cloning and Stem Cells·D Bousquet, P Blondin
Nov 18, 2005·Theriogenology·R Tayfur TecirliogluAndrew J French
Apr 27, 2010·Developmental Biology·S T BalbachM Boiani
Sep 12, 2013·Cellular Reprogramming·D N Wells
Oct 31, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Bingteng XieZhonghua Liu
Apr 6, 2018·Cellular Reprogramming·Naresh L SelokarSuresh K Singla
Aug 21, 2018·Animal Reproduction Science·Monika SainiSuresh K Singla

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Citations

Feb 20, 2020·Stem Cell Research & Therapy·Pengxiang QuEnqi Liu
Jan 23, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Yun FengFenghua Lu

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Methods Mentioned

BETA
acetylation
flow cytometry
PCR
confocal microscopy
biopsy
electrophoresis

Software Mentioned

SPSS
Flowjo Cycle Analysis
Leica LAX -

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