Successful control of aggregation and folding rates during refolding of denatured lysozyme by adding N-methylimidazolium cations with various N'-substituents

Biotechnology Progress
Satoshi YamaguchiTeruyuki Nagamune

Abstract

The present study aimed to obtain more effective refolding agents and to understand the influence of their chemical structures on their function as refolding agents. To achieve these aims, we investigated the effects of a large variety of N'-substituted N-methylimidazolium chlorides on the oxidative refolding of lysozyme in a high throughput manner. Among the molecules examined, N-methylimidazolium cations with a short N'-alkyl chain, such as an N'-ethyl or N'-butyl chain, significantly enhanced the refolding yield compared to conventional refolding additives such as arginine hydrochloride and Triton X-100. Detailed kinetic analyses revealed that the effective cations selectively decreased the aggregation rate constant (kA) without any large decreases in the folding rate constant (kN). However, when the hydrophobicity of the N'-substituent of the cations was increased, the desirable properties of the short N'-alkyl chain-type cations for protein refolding were diminished. Furthermore, increases in the N'-alkyl chain length to an N'-octyl or N'-dodecyl chain drastically decreased the kA values, thereby increasing the ratio of kN to kA, despite the very small kN values and resulting in enhanced refolding yields. Thus, by tuning t...Continue Reading

Citations

Feb 9, 2011·Applied Biochemistry and Biotechnology·Etsushi YamamotoTeruyuki Nagamune
Jul 8, 2015·MethodsX·Tasha B ToroTerry J Watt
Sep 22, 2010·Journal of Bioscience and Bioengineering·Etsushi YamamotoTeruyuki Nagamune
Jan 27, 2009·Biochemical and Biophysical Research Communications·Ryota AbeKouhei Tsumoto
Aug 30, 2008·Biophysical Chemistry·Makoto NakakidoKouhei Tsumoto
Oct 2, 2015·Chemical Reviews·Tamar L Greaves, Calum J Drummond
Feb 10, 2009·Chembiochem : a European Journal of Chemical Biology·Shinya Tsukiji, Teruyuki Nagamune
Dec 13, 2016·International Journal of Biological Macromolecules·Awanish KumarPannuru Venkatesu
Jul 15, 2017·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Nov 8, 2014·Physical Chemistry Chemical Physics : PCCP·P Madhusudhana ReddyP Venkatesu
Mar 8, 2018·Biophysics Reviews·Mouhamad Reslan, Veysel Kayser
Feb 28, 2019·Biotechnology Journal·Satoshi YamaguchiAkimitsu Okamoto
Sep 12, 2012·Biotechnology Journal·Satoshi YamaguchiTeruyuki Nagamune
Oct 18, 2013·Physical Chemistry Chemical Physics : PCCP·Angelo Miguel FigueiredoEurico J Cabrita
Sep 6, 2012·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Aug 10, 2013·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

© 2021 Meta ULC. All rights reserved