Abstract
An 11-yr, 9-mo-old girl with a 5-hr history of severe aplastic anemia unresponsive to androgen or steroid therapy was treated a bone marrow transplant from her 7-yr-old, HLA-MLC identical, APO incompatible, male sibling. Donor neutrophils were positive for the NA1 antigen. The patient's pretransplant serum contained a neutrophil specific antibody, anti-NA1, reactive against donor neutrophils, which was demonstrable by both granulocyte agglutination and granulocyte cytotoxicity assays. The transplant preparative regimen consisted of cyclophosphamide, 50 mg/kg/day for 4 days, and total lymphoid irradiation (750 rads, 26 rads/min by 4 meV linear accelerator). A two plasma volume exchange (4,800 ml) by continuous flow centrifugation with an Aminco cell separator was performed one day prior to transplant because of ABO incompatibility. Following plasma exchange, anti-NA1 cytotoxic titer, 1:8 preexchange, was no longer detectable; anti-NA1 agglutinating titer had only decreased from 1:64 to 1:32. She experienced no adverse reaction to transplantation of 4:8 X 10(8) nucleated cells/kg. Marrow engraftment was demonstrable by Day 14 by steadily increasing leukocyte and platelet counts, red cells of donor ABO group, and bone marrow chrom...Continue Reading
References
Jul 1, 1977·Transplantation·R P WarrenE D Thomas
Oct 1, 1978·Transplantation·C D BucknerE D Thomas
Sep 1, 1979·Transplantation·R A CliftB W Goodell
Jul 1, 1978·Vox Sanguinis·F W VerheugtC P Engelfriet
Jan 1, 1978·Vox Sanguinis·F W VerheugtC P Engelfriet
Jan 13, 1977·The New England Journal of Medicine·R StorbE D Thomas
Apr 17, 1975·The New England Journal of Medicine·E ThomasC D Buckner
Sep 1, 1975·Southern Medical Journal·A H Ragab
Apr 1, 1975·Journal of Immunological Methods·A F Jiang, P Lalezari
Jan 1, 1976·Vox Sanguinis·P R OlsonJ McCullough
Aug 1, 1974·Journal of Immunological Methods·D English, B R Andersen
May 1, 1972·The Journal of Pediatrics·L A BoxerP Lalezari
Jan 1, 1980·Annals of Internal Medicine·R StorbP L Weiden
Jan 1, 1980·British Journal of Haematology·C HershkoJ Fitchen
Citations
Jan 1, 1993·International Journal of Clinical & Laboratory Research·T R Klumpp
Sep 23, 2008·Bone Marrow Transplantation·T InukaiK Sugita
Mar 1, 1991·Transfusion Medicine·R L RodwellA H Waters
Jan 1, 1994·Vox Sanguinis·J McCullough
Jan 23, 2008·Vox Sanguinis·J Bux
Aug 1, 1988·Tissue Antigens·M J JagerJ J van Rood
Mar 4, 2009·Tissue Antigens·K Abou-ChakerA Salama
Jun 10, 2010·PloS One·John R Harp, Thandi M Onami
Aug 13, 2013·Expert Opinion on Investigational Drugs·Shigehiko MizutaniHiroshi Kobayashi
Sep 1, 1996·Photochemistry and Photobiology·J HoerterA Eisenstark
Oct 1, 1984·American Journal of Reproductive Immunology : AJRI·P R MadyasthaD H Levine
Mar 1, 1983·Human Pathology·J McCullough