Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4.

Journal of Experimental Botany
Karina Fabiana RibichichRaquel L Chan

Abstract

Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expe...Continue Reading

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Citations

May 1, 2020·GM Crops & Food·Mariana V ChiozzaPatricia V Miranda
Sep 10, 2020·Journal of Experimental Botany·R L ChanM E Otegui
Mar 14, 2021·Journal of Experimental Botany·María Florencia PerottiRaquel Lía Chan
May 26, 2021·Plant Physiology and Biochemistry : PPB·Marcos Fernando BassoMaria Fatima Grossi-de-Sa
Aug 14, 2021·Plant Biotechnology Journal·Min ZhangZhixi Tian
Jul 17, 2021·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Wenjing WangChen Huang
Oct 7, 2021·Physiologia Plantarum·José Eduardo Minussi WinckNereu Augusto Streck

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Datasets Mentioned

BETA
AF339748
AF339749

Methods Mentioned

BETA
transgenic
environmental stress
PCR
RNA-seq
electrophoresis

Software Mentioned

FastQC
ImageJ
featureCounts
agriGO
Trimmomatic
SPSS
R Core
INDEAR
DESeq2
MultiQC

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