Successful "in-flight" activation of natural killer cells during long-distance shipping.

Transfusion
Scott A KoepsellDavid H McKenna

Abstract

Natural killer (NK) cells have shown promise in the treatment of malignancy. However, the widespread use of these cells may be limited by both the lack of resources and the expertise needed to manufacture them and the apparent need to use only fresh cells. The NHLBI-sponsored Production Assistance for Cellular Therapies group was established to provide the resources and expertise to carry out cell therapy research, including support of clinical trials. Here we describe the qualification of in transit activation of an NK-cell therapy product in preparation for a Phase I clinical trial at a distant medical center. Nonmobilized apheresis mononuclear cell collections were CD3+ cell depleted, placed into culture bags with interleukin (IL)-2, and shipped from Minneapolis/Saint Paul, Minnesota, to Columbus, Ohio, and back to Minneapolis/Saint Paul, under warm, monitored temperatures. Products underwent quality control (QC) testing including cell count, immunophenotyping, viability, endotoxin, sterility culture, and cytotoxicity assays. One product tested the relative importance of IL-2 and controlled incubation. The length of shipment ranged from 14 to 16 hours, and temperatures were well controlled. QC testing was acceptable based up...Continue Reading

Citations

Dec 5, 2015·Frontiers in Immunology·Katayoun Rezvani, Rayne H Rouce
Feb 7, 2013·Transfusion·David R Shook, Wing Leung
Jun 8, 2012·Transfusion·Scott A KoepsellDavid H McKenna
Nov 13, 2015·Cancer Immunology, Immunotherapy : CII·Garnet SuckTorsten Tonn
Jul 17, 2018·International Journal of Immunogenetics·Jenna K Johnson, Jeffrey S Miller
Apr 3, 2019·Advanced Science·Rami El AssalUtkan Demirci
May 12, 2017·Frontiers in Immunology·Markus GranzinEvelyn Ullrich
Jul 6, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wing Leung
May 19, 2021·Scientific Reports·Jian GuFrederic Zenhausern

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