PMID: 11906923Mar 22, 2002Paper

Successful piglet production after transfer of blastocysts produced by a modified in vitro system

Biology of Reproduction
Kazuhiro KikuchiTakashi Nagai

Abstract

Porcine in vitro production (IVP) systems, including in vitro maturation (IVM) and in vitro fertilization (IVF) of oocytes and their subsequent in vitro culture (IVC), have been modified by many researchers, but are still at a low level because of a low developmental rate of embryos to the blastocyst stage and their poor qualities. Our objectives were to establish reliable IVP procedures for porcine blastocysts and to examine the ability of the blastocysts to develop to term after transfer to recipients. Porcine cumulus-oocyte complexes were matured in vitro under 5% O(2) or 20% O(2), fertilized in vitro under 5% O(2), and subsequently cultured under 5% O(2) in 1) IVC medium supplemented with glucose (IVC-Glu) from Day 0 (the day of IVF) to Day 6; 2) IVC-Glu from Days 0 to 2, then IVC medium supplemented with pyruvate and lactate (IVC-PyrLac) from Days 2 to 6; 3) IVC-PyrLac from Days 0 to 2, then IVC-Glu from Days 2 to 6; and 4) IVC-PyrLac from Days 0 to 6. There were no significant differences in blastocyst formation rates on Day 6 between the 5% O(2) and 20% O(2) conditions (19.9% and 14.0%, respectively). However, the quality of blastocysts, as evaluated by the total cell number, was better after IVM under 5% O(2) than under...Continue Reading

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Citations

Jul 28, 2011·Zygote : the Biology of Gametes and Early Embryos·Jung-Taek KangByeong-Chun Lee
Jan 30, 2007·Reproduction, Fertility, and Development·Gábor VajtaZoltán Macháty
May 9, 2008·Reproduction, Fertility, and Development·C AlmiñanaE A Martinez
Mar 10, 2012·Reproduction, Fertility, and Development·Tomomi MitoHiroyoshi Hoshi
Oct 26, 2002·Cloning and Stem Cells·Kazuhiro KikuchiHideaki Tojo
Apr 1, 2008·Cloning and Stem Cells·Mayuko KuromeHiroshi Nagashima
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Mar 23, 2011·The Journal of Reproduction and Development·Koji Yoshioka
May 25, 2011·The Journal of Reproduction and Development·Rentsenkhand SambuuJohanna Fink-Gremmels
Mar 28, 2012·The Journal of Reproduction and Development·Tamás SomfaiTakashi Nagai
Nov 9, 2005·The Journal of Reproduction and Development·Kazuhiro KikuchiNaomi Kashiwazaki
Oct 17, 2007·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima
Nov 21, 2007·The Journal of Reproduction and Development·Carlos Manuel Ulloa UlloKazuhiro Kikuchi
Feb 26, 2008·The Journal of Reproduction and Development·Tamás SomfaiKazuhiro Kikuchi
Apr 15, 2008·The Journal of Reproduction and Development·Koji YoshiokaAkira Onishi
May 21, 2008·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima

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