PMID: 9545584May 16, 1998Paper

Successful recovery of the normal electrophysiological properties of PorB (class 3) porin from Neisseria meningitidis after expression in Escherichia coli and renaturation

Biochimica Et Biophysica Acta
J SongM Colombini

Abstract

Neisseria meningitidis PorB class 3 porins obtained either from native membranes (wild-type) or recovered from inclusion bodies following expression in Escherichia coli (recombinant), have been reconstituted into solvent-free planar phospholipid membranes. The wild-type and recombinant porins exhibited the same single-trimer conductance (1-1.3 nS in 200 mM NaCl), tri-level closure pattern, characteristic of functional channel trimers, and pattern of insertion into planar membranes. Both proteins were open at low voltages and displayed two voltage-dependent closure processes, one at positive and the other at negative potentials. Both showed asymmetric voltage dependence such that one gating process occurred at lower voltages (Vo=15 mV) than the other (Vo=25 mV). The sign of the potential that resulted in closure at low voltages varied from membrane to membrane indicating that they may have the property of auto-directed insertion (in analogy to the mitochondrial channel, VDAC). In the case of the recombinant porin, the steepness of the voltage dependence of one gating process was slightly less (n=1.3) than that observed for the other process or for the wild-type channel (n=1.5-1.7). Both channels have a high (40%) probability of ...Continue Reading

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Citations

Feb 25, 2003·Trends in Microbiology·Paola MassariLee M Wetzler
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mikio TanabeT M Iverson
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·P MassariL M Wetzler
Nov 7, 2006·Journal of Bacteriology·Onkar Sharma, William A Cramer
Oct 26, 2012·The Biochemical Journal·Kornelius ZethThomas Rudel
Feb 18, 2004·Biochimica Et Biophysica Acta·Edson J MarquesOleg V Krasilnikov
Jun 2, 2009·Vaccine·Ian M Feavers, Mariagrazia Pizza
Apr 29, 2009·The International Journal of Biochemistry & Cell Biology·Lan WeiNicole A Beard
Oct 7, 2004·Biophysical Journal·Stanislav D ZakharovWilliam A Cramer
Jun 6, 2007·The Journal of Biological Chemistry·Onkar SharmaWilliam A Cramer
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer M BurkeLee M Wetzler
Feb 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paola MassariLee M Wetzler
May 23, 2006·Archives of Biochemistry and Biophysics·Conceição A S A Minetti, David P Remeta
Sep 16, 2021·Expert Review of Vaccines·Myron ChristodoulidesJohn E Heckels

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