PMID: 2492476Feb 1, 1989Paper

Successful reversal of diabetes in nude rats by transplantation of isolated adult human islets of Langerhans

Diabetes
S P LakeR F James

Abstract

A method is described in which the viability of isolated adult human islets of Langerhans can be assessed in vivo. The Rowett nude rat, made diabetic with streptozocin (STZ), has been used as the islet recipient in these studies. Although these animals are athymic and are able to accept xenogeneic grafts for prolonged periods, they are very susceptible to dehydration and infection once made diabetic. Therefore, a considerably shortened diabetes induction period was used. The basis of the study was to prepare pure adult human pancreatic islets that were cultured for 48 h. Nude rats were given 80 mg/kg i.v. STZ during islet isolation and were transplanted with 800-1000 islets under the renal capsule at 48 h. To monitor islet function, animals were bled regularly for random blood glucose measurements and were given a glucose tolerance test at day 20. The kidney containing the graft was removed on day 21 to allow histological assessment of the graft and to confirm that glucose control was due to the transplanted islets and was not secondary to reversion of the animal's own islets. Seven rats were transplanted, and five were deemed to have received viable human islets. Two rats that received islets from the same donor did not revers...Continue Reading

Citations

Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J M CashR L Wilder
Apr 9, 1998·Transplantation Proceedings·D BrandhorstR G Bretzel
Mar 17, 1999·Transplantation Proceedings·J Kovarik, T E Mandel
Mar 17, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·S A WhiteM L Nicholson
Jun 15, 1996·Transplantation·J H JuangG C Weir
Jun 22, 2005·Treatments in Endocrinology·Daniel M RidgwayRachel M Kimber
Nov 7, 2007·Endocrinology and Metabolism Clinics of North America·Paolo Fiorina, Antonio Secchi
Dec 24, 2005·Expert Opinion on Biological Therapy·Shinichi MatsumotoCamillo Ricordi
Oct 27, 2007·Expert Opinion on Biological Therapy·Simona MarzoratiCamillo Ricordi
Jan 5, 1999·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·L Rosenberg

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