Successful treatment of experimental diabetes by sequential transplantations of multiple-donor pancreatic islet allografts

Transplantation
T KanaiT Maki

Abstract

Fifty hand-picked islets were freshly prepared from DBA/2 mice and transplanted four times into a streptozotocin-induced diabetic B6AF1 mouse without immunosuppression. Blood sugar levels decreased progressively and all recipients became normoglycemic after the 4th grafting. Four repeated transplantations at 2-4-day or 14-day intervals restored normoglycemia for more than 200 days in virtually all recipients. However, a majority of recipients given four transplantations of 50 DBA/2 islets at 7-day intervals or four transplantations of 100 DBA/2 islets at 4-day or 14-day intervals acutely rejected their grafts. When handpicked islets were freshly prepared from each of four histoincompatible donors (DBA/2, DBA/1, A.SW, and C3H) and sequentially transplanted four times (islets from one donor for each transplantation) into diabetic B6AF1 recipients, virtually all recipients maintained normoglycemia for more than 200 days regardless of the number of islets per grafting or intervals between transplantations. Since the purity of human islet preparations to date has been at the level of crude-digested islets, crude islets were used in sequential transplantation. Four transplantations of approximately 50 crude islets prepared from each ...Continue Reading

Citations

Sep 1, 1993·In Vitro Cellular & Developmental Biology. Animal·C Y KuoH G Herrod

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.