Successful ultrarapid cryopreservation of wild Iberian ibex (Capra pyrenaica) spermatozoa

Theriogenology
J PradieeJ Santiago-Moreno

Abstract

A method for cryopreserving wild ibex sperm at high cooling rates was developed. To design a freezing solution based on Tris, citric acid, and glucose (TCG), two preliminary experiments were performed using glycerol (GLY) and dimethyl sulfoxide (DMSO) at different concentrations (5%, 10%, 20%). The 10% GLY + 10% DMSO combination reduced (P < 0.05) frozen-thawed sperm motility, which reached a minimum when 20% GLY + 20% DMSO was used. In the second experiment, sperm tolerance to three sucrose concentrations was evaluated (100-mM sucrose, 300-mM sucrose, 500-mM sucrose). Frozen-thawed sperm motility and sperm viability decreased (P < 0.05) at concentrations above 300 mM. The ultrarapid cooling procedure finally used involved a TCG egg yolk (ey)-based extender with 100-mM sucrose, either alone or with 5% GLY with or without BSA. Two warming procedures (37 °C vs. 60 °C) were also evaluated. The TCG ey with 100-mM sucrose but without GLY/BSA returned the best sperm quality variables. Slow warming at 37 °C strongly affected (P < 0.05) sperm motility and viability in all groups. Sperm selection by density gradient centrifugation produced no motile sperm when slow warming was performed. In contrast, when fast warming was used, sperm se...Continue Reading

References

Mar 1, 1989·Journal of Reproduction and Fertility·A T Canvin, M M Buhr
Oct 1, 1988·The Pediatric Infectious Disease Journal·J Siegel
Dec 1, 1995·British Poultry Science·K TselutinB Tur
Dec 22, 1999·Human Reproduction·L KuleshovaA Trounson
Jan 5, 2002·Theriogenology·S P Leibo, N Songsasen
Apr 12, 2005·Reproductive Biomedicine Online·Vladimir IsachenkoHans van der Ven
May 3, 2008·Expert Review of Medical Devices·Aniruddha BagchiJohn K Critser
May 17, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·E IsachenkoR Sánchez
Apr 20, 2010·Human Reproduction·L MareeG van der Horst
Apr 14, 2011·Andrologia·R SánchezE Isachenko
Oct 22, 2011·Animal Reproduction Science·M R Fernández-SantosJ Santiago-Moreno
Jan 28, 2012·Reproduction, Fertility, and Development·Vladimir IsachenkoRaul Sanchez
Mar 27, 2012·Theriogenology·S M HosseiniM H Nasr-Esfahani
Oct 23, 2012·Animal Reproduction Science·P Jiménez-RabadánA J Soler
Jun 19, 2013·Theriogenology·R LavaraM Baselga
Jun 4, 2014·Fertility and Sterility·Kai Mee WongSjoerd Repping

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Citations

Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·M Diaz-JimenezM Hidalgo
Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·C ConsuegraM Hidalgo
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·M Diaz-JimenezM Hidalgo
Oct 19, 2019·Reproduction in Domestic Animals = Zuchthygiene·Cesar ConsuegraManuel Hidalgo
Aug 23, 2020·Animals : an Open Access Journal From MDPI·Manuel HidalgoJesús Dorado
Jan 31, 2021·Animal : an International Journal of Animal Bioscience·M Diaz-JimenezM Hidalgo
Mar 23, 2021·Biopreservation and Biobanking·Maria Noel VieraRodolfo Ungerfeld
Aug 22, 2021·Reproduction in Domestic Animals = Zuchthygiene·Manuel Hidalgo

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