Succinate-mediated catabolite repression control on the production of glycine betaine catabolic enzymes in Pseudomonas aeruginosa PAO1 under low and elevated salinities

Microbiology
Farès DiabMohamed Jebbar

Abstract

Glycine betaine (GB) and its immediate precursors choline and carnitine, dimethylsulfonioacetate, dimethylsulfoniopropionate, ectoine and proline were effective osmoprotectants for Pseudomonas aeruginosa, but pipecolate, trehalose and sucrose had no osmoprotective effect. GB was accumulated stably or transiently when succinate or glucose, respectively, was used as a carbon and energy source. The catabolite repression mediated by succinate occurred at both low and high salinities, and it did not involve the global regulators Vfr and Crc. A proteomic analysis showed that at least 21 proteins were induced when GB was used as a carbon and energy source, and provided evidence that succinate repressed the synthesis of all these proteins. Many of the proteins induced by GB (sarcosine oxidase, serine hydroxymethyltransferase and serine dehydratase) are involved in GB catabolism. In addition, GB uptake was stimulated at high medium osmolalities but it was insensitive to catabolite repression by succinate. Despite its ability to inhibit betaine catabolism, succinate did not allow any better growth of P. aeruginosa cells under hyperosmotic constraint. Conversely, as observed for cells supplied with glucose, a transient accumulation of GB ...Continue Reading

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Citations

May 16, 2013·Extremophiles : Life Under Extreme Conditions·R Eric Collins, Jody W Deming
May 24, 2011·Applied and Environmental Microbiology·Liam F FitzsimmonsMatthew J Wargo
Sep 25, 2007·Applied and Environmental Microbiology·Emilie Dordet-FrisoniSabine Leroy
Jan 29, 2013·Applied and Environmental Microbiology·Matthew J Wargo
Dec 24, 2008·Infection and Immunity·Matthew J WargoDeborah A Hogan
Mar 5, 2013·Journal of Bacteriology·Benjamin R LundgrenChristopher T Nomura
Oct 8, 2013·Journal of Bacteriology·Ken J HampelMatthew J Wargo
Feb 15, 2007·Environmental Microbiology·Mohammed EssendoubiMohamed Jebbar
Oct 12, 2013·Chirality·Valerie VranovaPavel Formanek
Apr 29, 2015·Water Research·Muhammad Tariq KhanJean Philippe Croue
Feb 25, 2014·Applied and Environmental Microbiology·Abdallah BashirErhard Bremer
Mar 12, 2020·ChemSusChem·Mee-Rye ParkSteven W Singer
Mar 17, 2020·Applied Microbiology and Biotechnology·Yunhai ShaoWeihong Zhong
Jan 26, 2008·Journal of Proteome Research·Muriel Guyard-NicodèmeSylvie Chevalier

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