Succinate transport by free-living forms of Rhizobium japonicum.

Journal of Bacteriology
C F McAllister, J E Lepo

Abstract

We have demonstrated that the transport of succinate into the cells of Rhizobium japonicum strains USDA 110 and USDA 217 is severely inhibited by cyanide, azide, and 2,4-dinitrophenol, but not by arsenate. These results suggest an active mechanism of transport that is dependent on an energized membrane, but does not directly utilize ATP. The apparent Km for succinate was 3.8 microM for strain USDA 110 and 1.8 microM for strain USDA 217; maximal transport velocities were 1.5 and 3.3 nmol of succinate per min per mg of protein, respectively. The expression of the succinate uptake activity was inducible rather than constitutive, with succinate and structurally related compounds being the most effective inducers. The mechanism showed some specificity for succinate and similar organic acids; fumarate and L-malate were classical competitive inhibitors of the system. In general, the best competing compounds were also the best carbon substrates for induction of succinate uptake activity. EDTA inhibited the transport of succinate, implying a role for divalent cations in the system. When various divalent cations were used to reconstitute EDTA-inhibited activity, Ca2+ was most effective, followed by Mg2+, which restored activity at about ...Continue Reading

References

Sep 1, 1978·Archives of Biochemistry and Biophysics·W W Kay, M J Cameron
Jan 1, 1972·Journal of Bacteriology·G Martínez-De Drets, A Arias
Apr 1, 1974·Canadian Journal of Microbiology·G Martinez-de DretsM Rovira de Cutinella
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Jan 1, 1971·European Journal of Biochemistry·W W Kay, H L Kornberg
Aug 29, 1967·Biochimica Et Biophysica Acta·J F Bergersen, G L Turner
Sep 1, 1982·Journal of Bacteriology·A GardiolG Martínez-Drets
Mar 1, 1982·Journal of Bacteriology·G E de VriesA Quispel
Oct 1, 1981·Journal of Bacteriology·T M FinanD C Jordan
Jul 1, 1982·Applied and Environmental Microbiology·J E Urban, F B Dazzo
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C W RonsonJ G Robertson
Mar 26, 1982·Science·H H KeyserD F Weber

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Citations

Jun 1, 1989·Molecular Microbiology·O K YaroshT M Finan
May 1, 1994·World Journal of Microbiology & Biotechnology·S BatistaG Martínez-Drets
Oct 1, 1992·Archives of Environmental Contamination and Toxicology·A Fabra de PerettiA M Evangelista de Duffard
Jul 14, 2000·Journal of Bacteriology·S YurgelM L Kahn
Sep 18, 2004·FEMS Microbiology Reviews·Svetlana N Yurgel, Michael L Kahn
Jan 23, 2002·Biochimica Et Biophysica Acta·I G JanauschG Unden
Nov 1, 1984·Applied and Environmental Microbiology·D K Button
Jun 1, 1989·Journal of Bacteriology·D B Karr, D W Emerich
Apr 1, 1993·Journal of Bacteriology·J B Robinson, W D Bauer
Mar 1, 1989·Applied and Environmental Microbiology·H K Reding, J E Lepo
Sep 1, 1985·Microbiological Reviews·D K Button
Oct 1, 1990·Applied and Environmental Microbiology·I Hoelzle, J G Streeter
Jul 1, 1984·Journal of Bacteriology·P H Reibach, J G Streeter
May 30, 1989·Biochimica Et Biophysica Acta·M R O'Brian, R J Maier

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