PMID: 14415995Apr 1, 1960Paper

Succinic dehydrogenase and cytochrome oxidase activities in cell cultures

The Journal of Biophysical and Biochemical Cytology
I LESLIE, M YARNELL

Abstract

Succinic dehydrogenase and cytochrome oxidase have been assayed in permanent cell lines (HEP 1, HEP 2, and HLM), in short-term cultures of chick embryo heart cells, and in various tissues. Their activities in different cells are compared by relating them to deoxyribonucleic acid. They are very low in HEP 1, HEP 2, and HLM cells by comparison with the activities in any normal tissues examined. All the succinic dehydrogenase was shown to be located in the mitochondria of the permanent cell lines by staining with tetrazolium derivatives. Both enzymes were more active in tissues of 19-day chick embryos than in those of 11- or 14-day embryos. The increasing activities found during normal development were quickly curtailed or reversed when heart cells were grown as monolayer cultures. Digitonin-treated mitochondria produced preparations with much higher activities of cytochrome oxidase than untreated samples. Activities measured in this way were again very much lower in HEP 1, HEP 2, and HLM cells than in the normal tissues. From the derived ratio of cytochrome oxidase:succinic dehydrogenase, it was apparent that cytochrome oxidase is diminished to a greater extent than succinic dehydrogenase in both permanent cell lines and short-te...Continue Reading

References

Feb 24, 1956·Science·O WARBURG
Jul 1, 1957·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M NACHLASA M SELIGMAN
Apr 1, 1958·Experimental Cell Research·O SUSCHNYW RUCKER
Mar 17, 1959·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M J DAWKINS
May 29, 1959·Science·E GUTTES, S GUTTES
Aug 1, 1959·Experimental Cell Research·G KELLNERW RUCKER
Mar 20, 1951·The Journal of General Physiology·A E MIRSKY, H RIS

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Citations

Jan 1, 1977·Acta histochemica·A R Kennedy, J B Little
Jan 1, 1971·Progress in Histochemistry and Cytochemistry·P J Melivick
Sep 1, 1962·Experimental Cell Research·K HAGMULLER, I LESLIE
Mar 1, 1966·Experimental Cell Research·J A Dickson, I Leslie

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