PMID: 7011797Jan 1, 1981Paper

Succinic semialdehyde dehydrogenases of Escherichia coli: their role in the degradation of p-hydroxyphenylacetate and gamma-aminobutyrate

European Journal of Biochemistry
M I Donnelly, R A Cooper

Abstract

Two physically and genetically distinct forms of succinic-semialdehyde dehydrogenase have been identified in Escherichia coli B. The two enzymes could be separated by filtration on Sephadex G-150 and their apparent molecular weights were 200 000 and 97 000. The larger enzyme, which is specific for NADP, is induced by growth on gamma-aminobutyrate. Its induction is highly coordinated with that of gamma-aminobutyrate:2-oxoglutarate transaminase, the enzyme which initiates degradation of gamma-aminobutyrate. The smaller enzyme, which is induced by growth on p-hydroxyphenylacetate, has been purified to 98% homogeneity by affinity chromatography in conjunction with conventional methods. Under standard assay conditions this enzyme acts preferentially with NAD but reduces NADP at 15% of the rate observed for NAD, primarily because of a difference in Km. Apparent Km values for succinic semialdehyde and NAD are 13.3 +/- 1.3 microM and 33.7 +/- 1.4 microM, respectively. The subunit molecular weight was estimated to be 55 000, indicating that the native enzyme is dimeric. The NAD-dependent succinic-semialdehyde dehydrogenase is also induced by exposure of cells to exogenous succinic semialdehyde, a treatment which has no effect on the amo...Continue Reading

References

Jul 1, 1976·Journal of Bacteriology·V L Sparnins, P J Chapman
May 23, 1976·Biochemical and Biophysical Research Communications·M G Barbour, R C Bayly
Oct 1, 1974·Journal of Bacteriology·V L SparninsS Dagley
Apr 1, 1972·Journal of Bacteriology·S Dover, Y S Halpern
Jun 1, 1958·Journal of Bacteriology·F F NOE, W J NICKERSON

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Citations

Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Jul 19, 2002·Molecular Microbiology·Boris R Belitsky, Abraham L Sonenshein
Aug 29, 2012·Journal of Applied Microbiology·C Feehily, K A G Karatzas
Jun 1, 1992·FEMS Microbiology Reviews·P J Large
Jun 1, 2018·Applied Microbiology and Biotechnology·Marleen OtzenDick B Janssen
Nov 1, 1987·Biochimie·M I PrietoA Garrido-Pertierra
Jun 1, 1988·Biochimie·A Fernández-Briera, A Garrido-Pertierra
Apr 14, 1988·Biochimica Et Biophysica Acta·M SànchezA Garrido-Pertierra
Feb 19, 1986·Biochimica Et Biophysica Acta·M I Prieto-SantosA Garrido-Pertierra
Nov 1, 2005·EcoSal Plus·Larry Reitzer
Sep 1, 1986·Microbiological Reviews·R CuninV Stalon
Feb 1, 1988·Journal of Bacteriology·L E Marek, J M Henson
Mar 24, 1989·Biochimica Et Biophysica Acta·M SanchezA Garrido-Pertierra

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