Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

MSphere
Rebekah B GuevaraDavid J Bzik

Abstract

The glycosylated mucin domain of the Toxoplasma gondii cyst wall glycoprotein CST1 is heavily stained by Dolichos biflorus agglutinin, a lectin that binds to N-acetylgalactosamine. The cyst wall is also heavily stained by the chitin binding lectin succinylated wheat germ agglutinin (s-WGA), which selectively binds to N-acetylglucosamine-decorated structures. Here, we tracked the localization of N-acetylglucosamine-decorated structures that bind to s-WGA in immature and mature in vitro cysts. s-WGA localization was observed at the cyst periphery 6 h after the differentiation of the tachyzoite-stage parasitophorous vacuole. By day 1 and at all later times after differentiation, s-WGA was localized in a continuous staining pattern at the cyst wall. Coinciding with the maturation of the cyst matrix by day 3 of cyst development, s-WGA also localized in a continuous matrix pattern inside the cyst. s-WGA localized in both the outer and inner layer regions of the cyst wall and in a continuous matrix pattern inside mature 7- and 10-day-old cysts. In addition, s-WGA colocalized in the cyst wall with CST1, suggesting that N-acetylglucosamine- and N-acetylgalactosamine-decorated molecules colocalized in the cyst wall. In contrast to CST1, ...Continue Reading

References

Aug 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B J Luft, J S Remington
Sep 1, 1985·Infection and Immunity·H D WardM E Pereira
Feb 1, 1980·European Journal of Biochemistry·M MonsignyF Delmotte
Nov 14, 1997·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J C BoothroydS Tomavo
Dec 13, 2000·International Journal for Parasitology·A M TenterL M Weiss
Dec 19, 2000·Infection and Immunity·Y W ZhangL M Weiss
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·J JelenskaP Gornicki
Jul 23, 2002·Molecular Biology of the Cell·Corinne MercierMarie-France Cesbron-Delauw
Nov 16, 2007·Nucleic Acids Research·Bindu GajriaBrian P Brunk
Feb 17, 2009·International Journal for Parasitology·Louis M Weiss, Jitender P Dubey
Jun 10, 2010·PLoS Neglected Tropical Diseases·Araceli Castillo-RomeroJose Manuel Hernandez
Aug 26, 2010·Journal of Visualized Experiments : JoVE·Crystal TobinLaura Knoll
Apr 14, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rima McLeodUNKNOWN Toxoplasmosis Study Group
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Dec 5, 2012·PLoS Pathogens·J Philip NanceEmma H Wilson
Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joshua B RadkeMichael W White
Oct 8, 2013·Eukaryotic Cell·John SamuelsonPhillips W Robbins
Nov 1, 2013·BioMed Research International·M Herrera-MartínezP Talamás-Rohana
Dec 31, 2014·Journal of Visualized Experiments : JoVE·Anita A Koshy, Carla M Cabral
May 20, 2015·Current Opinion in Microbiology·Christopher M West, Ira J Blader
Sep 26, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Samuel L HutsonRima McLeod

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
Fluorescence
glycosylation
GTPase
transgenic

Software Mentioned

Excel
Prism
Fiji
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.