PMID: 8942980Nov 26, 1996Paper

Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes

Proceedings of the National Academy of Sciences of the United States of America
A C Porchia, Graciela L Salerno

Abstract

Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrose-phosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium. Two SPS activities (SPS-I and SPS-II) were isolated by ion-exchange chromatography and partially purified. Four remarkable differences between SPSs from Anabaena and those from higher plants were shown: substrate specificity, effect of divalent cations, native molecular mass, and oligomeric composition. Both SPS-I and SPS-II accept Fru-6-P (K(m) for SPS-I = 0.8 +/- 0.1 mM; K(m) for SPS-II = 0.7 +/- 0.1 mM) and UDP-Glc as substrates (K(m) for SPS-I = 1.3 +/- 0.4 mM; K(m) for SPS-II = 4.6 +/- 0.4 mM), but unlike higher plant enzymes, they are not specific for UDP-Glc. GDP-Glc and TDP-Glc are also SPS-I substrates (K(m) for GDP-Glc = 1.2 +/- 0.2 mM and K(m) for TDP-Glc = 4.0 +/- 0.4 mM), and ADP-Glc is used by SPS-II (K(m) for ADP-Glc = 5.7 +/- 0.7 mM). SPS-I has an absolute dependence toward divalent metal ions (Mg2+ or Mn2+) for catalytic activity, not found in plants. A strikingly smaller native molecular mass (between 45 and 47 kDa) was determined by g...Continue Reading

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Citations

Mar 7, 2008·Current Microbiology·Ravi K AsthanaSureshwar P Singh
Apr 12, 2005·Plant Physiology and Biochemistry : PPB·Paula DesplatsGraciela L Salerno
Jun 8, 2002·Phytochemistry·Astrid Wingler
Feb 1, 2003·Gene·John E Lunn
Feb 25, 2003·Trends in Plant Science·Graciela L Salerno, Leonardo Curatti
Apr 16, 2002·Plant Physiology·John Edward Lunn
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·John C Meeks, Jeff Elhai
Aug 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Leticia L Torres, Graciela L Salerno
Dec 15, 2015·Biotechnology Advances·Katharina SchmölzerBernd Nidetzky
Apr 27, 2013·FEBS Letters·Giselle M A Martínez-NoëlGraciela L Salerno
Dec 15, 2015·FEMS Yeast Research·Wesley Leoricy MarquesAndreas Karoly Gombert
Feb 26, 2016·Environmental Microbiology·María A Kolman, Graciela L Salerno
Nov 9, 2010·Environmental Microbiology·Stephan Klähn, Martin Hagemann
Apr 8, 2015·Applied Microbiology and Biotechnology·Margo DiricksTom Desmet
Jul 14, 2010·FEMS Microbiology Reviews·Martin Hagemann
Jun 9, 2014·Plant Science : an International Journal of Experimental Plant Biology·Macarena Perez-Cenci, Graciela L Salerno
Nov 8, 2013·Molecular Plant·Elisabeth SchmidtmannIris Finkemeier
May 6, 2021·Journal of Experimental Botany·Carlos M FigueroaAlberto A Iglesias
Mar 13, 2014·Plant Physiology·Jaime M AmezagaMichael R Templeton

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