Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

Journal of Mass Spectrometry : JMS
Andrew D PalmerJosephine Bunch

Abstract

Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-µm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections.

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Citations

Mar 22, 2013·Analytical and Bioanalytical Chemistry·Rory T Steven, Josephine Bunch
Mar 5, 2013·Journal of the American Society for Mass Spectrometry·Rory T StevenJosephine Bunch
May 28, 2013·Journal of the American Society for Mass Spectrometry·Rory T StevenJosephine Bunch
May 14, 2014·Journal of the American Society for Mass Spectrometry·David M G AndersonKevin L Schey
Nov 14, 2015·Foods·John W ErdmanMartha Neuringer
Jul 31, 2012·Journal of Proteomics·Brendan Prideaux, Markus Stoeckli
Dec 10, 2014·Analytical Chemistry·Bernhard Spengler
Nov 21, 2015·Proteomics. Clinical Applications·Hannah E BowreyZsolt Ablonczy
Jul 25, 2013·Analytical Chemistry·Rian L GriffithsJosephine Bunch
Jul 31, 2018·Expert Review of Proteomics·Viviana GrecoAndrea Urbani
Oct 28, 2019·Journal of Mass Spectrometry : JMS·Berin A BoughtonAngus C Grey
Jul 7, 2020·Journal of the American Society for Mass Spectrometry·David M G AndersonKevin L Schey

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