Sucrose hydrolysis by thermostable immobilized inulinases from aspergillus ficuum

Enzyme and Microbial Technology
M Ettalibi, J C. Baratti

Abstract

The possibility of using thermostable inulinases from Aspergillus ficuum in place of invertase for sucrose hydrolysis was explored. The commercial inulinases preparation was immobilized onto porous glass beads by covalent coupling using activation by a silane reagent and glutaraldehyde before adding the enzyme. The immobilization steps were optimized resulting in a support with 5,440 IU/g of support (sucrose hydrolysis) that is 77% of the activity of the free enzyme. Enzymatic properties of the immobilized inulinases were similar to those of the free enzymes with optimum pH near pH 5.0. However, temperature where the activity was maximal was shifted of 10 degrees C due to better thermal stability after immobilization with similar activation energies. The curve of the effect of sucrose concentration on activity was bi-phasic. The first part, for sucrose concentrations lower than 0.3 M, followed Michaelis-Menten kinetics with apparent K(M) and Vm only slightly affected by immobilization. Substrate inhibition was observed at values from 0.3 to 2 M sucrose. Complete sucrose hydrolysis was obtained for batch reactors with 0.3 and 1 M sucrose solutions. In continuous packed-bed reactor 100% (for 0.3 M sucrose), 90% (1 M sucrose) or 8...Continue Reading

References

Mar 1, 1977·Biotechnology and Bioengineering·P A DickensheetsG T Tsao
Jan 1, 1992·Applied Biochemistry and Biotechnology·J S Melo, S F D'Souza
Jan 1, 1990·Journal of Chemical Technology and Biotechnology·A K GuptaR Singh
Jan 1, 1990·Agricultural and Biological Chemistry·M Ettalibi, J C Baratti
Jan 1, 1988·Methods in Enzymology·P Monsan, D Combes
Jan 1, 1973·Biochimie·J Boudrant, C Cheftel
Jan 1, 1983·Advances in Applied Microbiology·E J Vandamme, D G Derycke
May 15, 1981·Analytical Biochemistry·F N Onyezili, A C Onitiri
May 1, 1997·Applied Microbiology and Biotechnology·A Krastanov
Sep 1, 1997·Biomaterials·F SelampinarL Toppare
Oct 26, 1999·Applied Biochemistry and Biotechnology·L M Arruda, M Vitolo
Oct 26, 1999·Applied Biochemistry and Biotechnology·A PandeyJ D Fontana
Feb 15, 2000·Journal of Biomaterials Science. Polymer Edition·S AlkanY Hepuzer
Apr 1, 1984·Biotechnology and Bioengineering·P Monsan, D Combes
Jul 1, 1984·Biotechnology and Bioengineering·P MonsanI Alemzadeh

❮ Previous
Next ❯

Citations

Mar 30, 2011·Applied Biochemistry and Biotechnology·Gonçalo L M SantaPedro Fernandes
Aug 13, 2011·Bioprocess and Biosystems Engineering·Aline RichettiHelen Treichel
Oct 22, 2008·Applied Biochemistry and Biotechnology·Daniel Luciano FalkoskiSebastião Tavares de Rezende
Feb 20, 2013·New Biotechnology·Susana M PaixãoLuís Alves
Jun 30, 2015·Chemical Reviews·Angeles FarránRobert J Linhardt
Apr 22, 2015·Journal of Industrial Microbiology & Biotechnology·Mariusz TrytekMarcin Skowronek
Nov 29, 2014·Journal of Biosciences·Tarek M MohamedAly Sayed Aly
Jan 25, 2013·International Journal of Molecular Sciences·Fabiano Jares ContesiniHélia Harumi Sato
Sep 17, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tais B GarletRaquel C Kuhn
Dec 14, 2017·Critical Reviews in Biotechnology·Gerard NeerajVaidyanathan Vinoth Kumar
Jun 12, 2009·Critical Reviews in Biotechnology·Krishnan VijayaraghavanN Sravya Sowdamini
Jun 20, 2014·Microbial Cell Factories·Duniesky MartínezLázaro Hernández

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.